Figure 5From: Perturbing the metabolic dynamics of myo-inositol in developing Brassica napus seeds through in vivo methylation impacts its utilization as phytate precursor and affects downstream metabolic pathwaysHPLC evidence of ononitol production by the IMT transgene in transgenic lines. (A) Partial trace of mature seed sugar analysis from a representative transgenic line. The peak at 6.75 minutes corresponds to ononitol; (B) quantitative levels of ononitol in mature seeds of different phaseolin-IMT transgenic lines (Ph1-18, Ph2-15 and Ph3-19). (C) IMT enzyme assay of IMT transgenic lines (T) vs. wild-type control (W). Relative peak areas represent the average ±SE (standard error) of three biological replicates.Back to article page