Gene expression analysis of napin- IMT and phaseolin- IMT transgenic lines. (A) Gene expression analysis of developing seeds of phaseolin-IMT transgenic lines Ph1-18, Ph2-15 and Ph3-19 at 15, 20, 25, 30, 35 and 40 days after pollination (DAP). (B) Gene expression analysis of midrange seed development (25 DAP) of the napin-IMT transgenic B. napus line, N-11. Panels a-c, Western analysis, Northern analysis and RNA gel. Rec, Histidine tagged recombinant IMT produced in E. coli; Ice, ice plant protein extract; W, Protein extract from Westar.