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Figure 4 | BMC Plant Biology

Figure 4

From: Perturbing the metabolic dynamics of myo-inositol in developing Brassica napus seeds through in vivo methylation impacts its utilization as phytate precursor and affects downstream metabolic pathways

Figure 4

Gene expression analysis of napin- IMT and phaseolin- IMT transgenic lines. (A) Gene expression analysis of developing seeds of phaseolin-IMT transgenic lines Ph1-18, Ph2-15 and Ph3-19 at 15, 20, 25, 30, 35 and 40 days after pollination (DAP). (B) Gene expression analysis of midrange seed development (25 DAP) of the napin-IMT transgenic B. napus line, N-11. Panels a-c, Western analysis, Northern analysis and RNA gel. Rec, Histidine tagged recombinant IMT produced in E. coli; Ice, ice plant protein extract; W, Protein extract from Westar.

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BMC Plant Biology

ISSN: 1471-2229

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