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Table 2 Responses of gene-silenced leaf disks to abiotic stresses, and bacterial pathogen growth on silenced plants

From: A high-throughput virus-induced gene silencing protocol identifies genes involved in multi-stress tolerance

Name of silenced gene Abiotic stresses1 Disease resistance2(bacterial pathogens)
Host Nonhost
Salinity High temperature Low temperature Oxidative Osmoticum Pstab PstT1 XCV
WT * * * * * ## No No
TRV::GFP * * * * * ## No No
NbSOS1 ++++ * * + * ## No
NbAPX * ++ + +++ * ## No
NbGST ++ + * ++ ++ ###
NbCAT3 ++ * * ++ * ###
NbDHAR + + * ++ * ## No
NbGPX ++ * * ++++ * ## No
NbFER2 * * * * * ## No
NbHSP101 + ++++ * ++ * ## No
NbBIP5 * + ++ * * ## No No
NbP5CS1 ++ + + +++ ++++ ### No
NbCBL1 * + * + * ## No No
NbMYC2 ++ ++ * + * ### No
NbGBP16 * * * * * ## No No
NbRBX1 * + * + ++ ## No No
NbPAL1 * ++ + ++ * ## No
NtEDS1 * ++ * * ++++ ###
NtNPR1 ++ ++ * + * ### No
NtRAR1 ++ + * + * ###
NbADR1 * + * + +++ ###
NbMC + ++ * * * ## No No
NbCYCD2 ++ * * ++ * ###
NbCTR1 + * * ++ * ## No
NbWRKY1 ++ ++ + * + ### No
NtMEK1 ++ + * ++ +++ ## No No
NbWRKY2 * ++ * * + ## No No
NbETR1 * ++ * + ++ ## No No
NbFLS * + * ++ * ## No
NbMYB1 ++ ++ * + ++ ## No No
  1. 1Abiotic stress phenotype was represented as *leaf disks with no observable phenotypic changes when compared to wild-type; +trivial discoloration; ++moderate discoloration with wrinkling; +++complete discoloration and/or wrinkle with necrotic spots and ++++complete cell death.
  2. 2The disease symptom development on pathogen-inoculated leaves was scored as percent leaf area covered and represented as ##50% of the leaf area showing disease symptoms when inoculated with host pathogen Pstab (Pseudomonas syringae pv. tabaci); ###75% of the leaf area showing disease symptoms when inoculated with Pstab; Noleaves with no visible disease symptoms when inoculated with nonhost pathogens PstT1 (P. syringae pv. tomato T1) and XCV (Xanthomonas campestris pv. vesicatoria); up to 50% of leaf showing disease symptoms when inoculated with PstT1 and XCV.