A high-throughput virus-induced gene silencing protocol identifies genes involved in multi-stress tolerance

BMC Plant Biology

Table 2 Responses of gene-silenced leaf disks to abiotic stresses, and bacterial pathogen growth on silenced plants

Name of silenced gene	Abiotic stresses¹					Disease resistance²(bacterial pathogens)
	Abiotic stresses¹					Host	Nonhost
	Salinity	High temperature	Low temperature	Oxidative	Osmoticum	Pstab	PstT1	XCV
WT	*	*	*	*	*	##	No	No
TRV::GFP	*	*	*	*	*	##	No	No
NbSOS1	++++	*	*	+	*	##	No	†
NbAPX	*	++	+	+++	*	##	†	No
NbGST	++	+	*	++	++	###	†	†
NbCAT3	++	*	*	++	*	###	†	†
NbDHAR	+	+	*	++	*	##	No	†
NbGPX	++	*	*	++++	*	##	†	No
NbFER2	*	*	*	*	*	##	†	No
NbHSP101	+	++++	*	++	*	##	No	†
NbBIP5	*	+	++	*	*	##	No	No
NbP5CS1	++	+	+	+++	++++	###	†	No
NbCBL1	*	+	*	+	*	##	No	No
NbMYC2	++	++	*	+	*	###	†	No
NbGBP16	*	*	*	*	*	##	No	No
NbRBX1	*	+	*	+	++	##	No	No
NbPAL1	*	++	+	++	*	##	†	No
NtEDS1	*	++	*	*	++++	###	†	†
NtNPR1	++	++	*	+	*	###	†	No
NtRAR1	++	+	*	+	*	###	†	†
NbADR1	*	+	*	+	+++	###	†	†
NbMC	+	++	*	*	*	##	No	No
NbCYCD2	++	*	*	++	*	###	†	†
NbCTR1	+	*	*	++	*	##	†	No
NbWRKY1	++	++	+	*	+	###	†	No
NtMEK1	++	+	*	++	+++	##	No	No
NbWRKY2	*	++	*	*	+	##	No	No
NbETR1	*	++	*	+	++	##	No	No
NbFLS	*	+	*	++	*	##	No	†
NbMYB1	++	++	*	+	++	##	No	No

¹Abiotic stress phenotype was represented as ^*leaf disks with no observable phenotypic changes when compared to wild-type; ⁺trivial discoloration; ⁺⁺moderate discoloration with wrinkling; ⁺⁺⁺complete discoloration and/or wrinkle with necrotic spots and ⁺⁺⁺⁺complete cell death.
²The disease symptom development on pathogen-inoculated leaves was scored as percent leaf area covered and represented as ^##50% of the leaf area showing disease symptoms when inoculated with host pathogen Pstab (Pseudomonas syringae pv. tabaci); ^###75% of the leaf area showing disease symptoms when inoculated with Pstab; ^Noleaves with no visible disease symptoms when inoculated with nonhost pathogens PstT1 (P. syringae pv. tomato T1) and XCV (Xanthomonas campestris pv. vesicatoria); ^†up to 50% of leaf showing disease symptoms when inoculated with PstT1 and XCV.

ISSN: 1471-2229