PT-Flax (phenotyping and TILLinG of flax): development of a flax (Linum usitatissimumL.) mutant population and TILLinG platform for forward and reverse genetics

Table 3 Characteristics of mutations in CAD and C3H amplicons

Target	Amplicon size	Identified mutations	Mutation frequency	Exon size	% of GC in exons	Identified mutations in exon	Type of mutation (exon)			% Mutation saturation
							Silent	Missense	Truncation
CAD	869	76	1/40 Kb	620	50.0	67	15	51	1	22.5
							(22.4%)	(76.1%)	(1.5%)
C3H	981	79	1/44 Kb	870	52.53	74	24	43	7	15.35
							(32.4%)	(58.1%)	(9.5%)

Silent mutations do not modify amino acids, missense mutations induce amino-acid modifications, truncation mutations induce a premature STOP codon in the reading frame. Percentage mutation saturation is calculated at the protein level as the number of amino acids modified compared to the total number of amino acids that can be potentially modified by EMS action.

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