Table 2 Locations and amplifications of primers in different cultivars with PINA-null allele
Primer | Position (Reference to ATG of Pinaor Pinb) | Expected PCR product amplified | |||||
|---|---|---|---|---|---|---|---|
Forward primer | Reverse primer | Chinese Spring | Yunong 202 | Dahongmang/Hulutou | Zuantoubaike | Yumai/Changyaomai/Yangqingke | |
Pina-1 | −10386a | −9170 | Yesc | Yes | Yes | Yes | Yes |
Pina-2 | −9189 | −8543 | Yes | Yes | Yes | Yes | Yes |
Pina-3 | −8951 | −8062 | Yes | Yes | Yes | Yes | Yes |
Pina-4 | −9065 | −8030 | Yes | Yes | Yes | Yes | Yes |
Pina-5 | −8332 | −7754 | Yes | Yes | Yes | Yes | No |
Pina-6 | −7896 | −7385 | Yes | Yes | Yes | Yes | No |
Pina-7 | −7748 | −7028 | Yes | Yes | Yes | Yes | No |
Pina-8 | −6044 | −5438 | Yes | Yes | Yes | Yes | No |
Pina-9 | −5850 | −4707 | Yes | Yes | Yes | Yes | No |
Pina-10 | −4168 | −3146 | Yes | Yes | Yes | No | No |
Pina-11 | −2225 | −1625 | Yes | Yes | Yes | No | No |
Pina-12 | −950 | 0 | Yes | Yes | Yes | No | No |
Pina-13 | −781 | −30 | Yes | Yes | Yes | No | No |
Pina-D | −40 | +474 | Yes | Yes | No | No | No |
Pina-14 | +654 | +1340 | Yes | Yes | No | No | No |
Pina-15 | +1116 | +2118 | Yes | Yes | No | No | No |
Pina-16 | +3229 | +3851 | Yes | Yes | No | Yes | No |
Pina-17 | +3835 | +4346 | Yes | Yes | No | Yes | No |
Pina-18 | 4283 | +4821 | Yes | Yes | No | Yes | No |
Pina-19 | +5116 | +5740 | Yes | Yes | Yes | Yes | No |
Pina-20 | +8439 | +9400 | Yes | Yes | Yes | Yes | No |
Pina-21 | +10695 | +11585 | Yes | Yes | Yes | Yes | No |
Pina-22 | +11560 | +12466 | Yes | Yes | Yes | Yes | No |
Pina-23 | +12260 | +13373 | Yes | Yes | Yes | Yes | No |
Pina-24 | +13464 | +14448 | Yes | Yes | Yes | Yes | No |
Pina-25 | +14435 | +15475 | Yes | Yes | Yes | Yes | No |
Pinb-1 | +15380 (−2766b) | +16330 (−1816) | Yes | Yes | Yes | Yes | No |
Pinb-2 | +16630 (−1516) | +17387 (−759) | Yes | Yes | Yes | Yes | No |
Pinb-3 | (−779) | (−253) | Yes | Yes | -d | - | No |
Pinb-4 | (−407) | (+121) | Yes | Yes | - | - | No |
Pinb-D | (−52) | (+542) | Yes | Yes | - | - | No |
Pinb-5 | (+1024) | (+2554) | No | No | - | - | No |
Pinb-6 | (+2484) | (+3612) | Yes | Yes | - | - | No |
Pinb-7 | (+3539) | (+4480) | Yes | Yes | - | - | No |
Pinb-8 | (+4425) | (+5353) | Yes | Yes | - | - | No |
Pinb-9 | (+5275) | (+6475) | Yes | Yes | - | - | No |
Pinb-10 | (+6460) | (+7574) | Yes | Yes | - | - | Yes |
Pinb-11 | (+7427) | (+8884) | Yes | Yes | - | - | Yes |
Pinb-12 | (+8819) | (+10319) | Yes | Yes | - | - | Yes |
Pinb-13 | (+10321) | (+11447) | Yes | Yes | - | - | Yes |