Figure 4

Proteins of CTLH complexes copurified with AtRanBPM from Arabidopsis extracts. A- Affinity purification of GFP-AtRanBPM from the input of S20 of GFP-AtRanBPM culture (IN) using GFP-Trap A beads. Proteins were eluted from affinity beads (IP: anti-GFP) and run on SDS gel electrophoresis with silver staining detection. MALDI-MS analysis identified among proteins copurified with AtRanBPM the plant homologues of proteins that form CTLH complex in human cells. B- Comparison of proteins copurified with AtRanBPM (numbers in brackets present number of band shown in Figure 4A) with their counterparts - the proteins of human CTLH complex.