Figure 1
Amylopectin and amylose structure of Wt and sbe1a mutant starch samples by HPSEC analysis. A. Proportions of chains1 from debranched2 β-dextrins during time course of β-amylolysis of amylopectin from Wt (----) and sbe1a mutant (- - -) starch using β-amylase (250 U/mL). B. Chromatograms1 of isoamylase-debranched and isoamylase-plus-pullulanase-debranched β-limit dextrins3 from amylose fraction from Wt and sbe1a mutant starch. 1Chromatographic regions were divided as in [40]. Proportions of DP ≥ 18, DP 8-17, DP 5-7, DP 4, DP 3 and DP 2 were calculated as the areas for DP ≥ 17.5, 7.5 ≤ DP ≤ 17.5, 4.5 ≤ DP ≤ 7.5, 3.5 ≤ DP ≤ 4.5, 2.5 ≤ DP ≤ 3.5, and DP ≤ 2.5, respectively, as in [40]. Proportions of chains in each region for B are presented in Additional File 4. Calculation was based on representative chromatograms for starch from one biological replication. Values are percentage by weight. 2Debranching was performed successively with isoamylase for 24 h and pullulanase for 24 h. 3β-Limit dextrin was obtained after 3 times of 24-h β-amylolysis on amylose.