Figure 6

The effect of selected fractions of EIBMPs on wall hydration in walls containing GvP1 network. Where A) GvP1 network (ca. 70% deposition) was formed in the presence of total endogenous EIBMPs (T2) and B), GvP1 network (ca. 60% deposition) was formed with pure GvP1 and GvEP1, i.e. in the absence of other, endogenous EIBMPs (T3). In all cases, following extensin network formation, residual monomeric extensin was removed from walls by saline extraction prior to the addition of selected protein fractions. All measurements were made and expressed as described in Figure 5. Key: EIBMP^a = endogenous levels of whole EIBMPs; EIBMP^c = GvP1-free EIBMPs fractionated from native cell walls; EIBMP^d = GvP1-depleted EIBMPs from H₂O₂-incubated cell walls (see methods); EIBMP^e = Medicago leaf extracellular, ionically binding matrix proteins; DTT = dithiothreitol. Figure legend text.