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Figure 3 | BMC Plant Biology

Figure 3

From: Extensin network formation in Vitis vinifera callus cells is an essential and causal event in rapid and H2O2-induced reduction in primary cell wall hydration

Figure 3

H 2 O 2 causes insolubilisation of the grapevine extensin, GvP1. A) Superose-12 chromatography of EIBMPs (saline eluates of 35 mg (FW) cells) from untreated cells (trace control), cells incubated with 100 mM H2O2 (trace + H2O2). A chromatogram of pure GvP1 is also indicated as a reference. A time course assay of GvP1 insolubilisation in muro is depicted in the inset. B) SP-Sepharose chromatography of whole EIBMPs. Fractions enriched in GvP1 and subject to TCA fractionation for the purification of GvP1 are delimited by solid grey lines.

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BMC Plant Biology

ISSN: 1471-2229

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