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Table 5 Butte 86 gamma gliadins identified by MS/MS from wheat flour.

From: Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour

Butte 86 contig # Band # 1 Total # peptides 2 % Coverage3 Unique peptides4 Enzyme used to generate peptide5
2 126 14 36.0 SQQQQVGQGSL CH
     QQLPQPQQPQQSFPQQQR CH
     SQQQQVGQGSLVQGQGIIQPQQPAQL CH
     NIQVDPSGQVQWLQQQLVPQLQQPL CH
     LQQPQQPFPQPQQQLPQPQQPQQ TH
4 188 22 56.5 SIIMQQEQRQGVQIRRPL CH
     LQPQQPQQSFPQQQQPL CH
     VSPDCSTINAPF CH
     VSPDCSTINAPFASIVVGIGGQ CH
     LQPQQPQQSFPQQQQPLIQL CH
     LQPQQPQQSFPQQQQPLIQLSL CH
     CQQPQQTIPQPHQTF CH
     IIMQQEQRQG TH
     IIMQQEQRQGVQ TH
     LQPQQPQQSFPQQQQPLIQ TH
     VDPGYQVHWPQQQPFPQPQQP TH
     VHWPQQQPFPQPQQP TH
     NFLLQQCNPVSLVSSLISMILPR TR
5 145 31 44.8 VPPECSIIRAPF6 CH
     IQPSLQQR CH
     IQPSLQQRL CH
     SQQQQLGQGTL6 CH
     QSFPQQQRPF CH
     ILLPLSQQQQL6 CH
     TQQPQQPFPQFQQPHQPF6 CH
     VQGQGIIQPQQLAQLEAIRSL6 CH
     SQQQQLGQGTLVQGQGIIQPQQL6 CH
     SQQQQLGQGTLVQGQGIIQPQQLAQLEAIRSL6 CH
     ILLPLSQQQQLGQGTL6 CH
     SQQQQLGQGTLVQGQGIIQPQQLAQL6 CH
     SQQPQQAFPQPQQTFPHQPQQQVPQPQQPQQPF CH
     HQPQQQFPQPQQPQQSFPQQQRPF CH
     HQPQQQFPQPQQPQQSFPQ CH
     AFPQPQQTFPHQPQQQVPQPQQPQQPF TH
     FHQPQQQFPQPQQPQQ TH
     FHQPQQQFPQPQQPQQSFPQQQRP TH
     IQPSLQQR TR
     QLAQLEAIR TR
     PFIQPSLQQR TR
     QSFPQQQRPFIQPSLQQR TR
6 186 16 36.5 RQPQQPF CH
     ASIVAGISGQ CH
     YQQPQQTFPQPQ CH
     LAQIPRQ TH
     IQILRPLFQ TH
     IIQPQQPAQYEVIRS TH
     FRQPQQPFY TH
     IIQPQQPAQYE TH
     YQQPQQTFPQPQQ TH
     FRQPQQPFYQQPQQTFPQPQQ TH
     FYQQPQQTFPQPQQ TH
7 170 13 43.4 LQPHQPF CH
     ASIVASIGGQ CH
     QGVQILVPL CH
     SQQQQVGQGIL CH
     SQQQQVGQGILVQGQGIIQPQQPAQL CH
     VYVPPYCST TH
     LQPHQPFSQQPQQ TH
     APFASIVASIGGQ TR
     QPFPQQPQQPYPQQPQQPFPQTQQPQQPFPQSK TR
11 149 4 27.6 YQQQQVGQGTLVQGQGIIQPQQPAQL CH
     SFPQQQPPF TH
17 180 7 24.9 ASIVADIGGQ CH
87    22.0 KAPFASIVADIGGQ CH
     APFASIVADIGGQ TR
38 169 16 37.5 ANIDAGIGGQ CH
108    39.7 GIIQPQQPAQLEGIRSLVL CH
     VPPNCSTINVPY CH
     VPPNCSTINVPYANIDAGIGGQ6 CH
     VTILRPLFQ6 TH
     INVPYANIDAGIGGQ TH
     NFLLQQCNHVSLVSSLVSIILPR TR
  1. 1 detailed in Vensel et al. (in preparation).
  2. 2 obtained by interrogation of "subset" database with MS/MS data.
  3. 3 excluding 19 amino acid signal peptide.
  4. 4 sequences of peptides obtained by MS/MS that are not shared by other Butte 86 gamma gliadins. Peptide containing the extra cysteine is indicated in italics.
  5. 5 CH, chymotrypsin; TH, thermolysin; TR, trypsin.
  6. 6 peptides that distinguish Butte 86 sequence from closest NCBI match.
  7. 7 MS/MS data consistent with either Butte 86 gamma gliadin #1 or #8.
  8. 8 MS/MS data consistent with either Butte 86 gamma gliadin #3 or #10.