Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour

Table 4 Evidence for expression of sequences encoding gamma gliadins with odd numbers of cysteine residues.

Protein accession #	Source	# Cysteine residues	Region of missing/additional cysteine	Reference	Sequence used to search NCBI¹	# ESTs in NCBI ²
[GenBank: CAC94868.1]	T. aestivum cv. Mjoelner	7	III	Arentz-Hansen et al. [18]	DCQVMRQQSCQQLAQIP	1
[GenBank: ACJ03505.1]	Aegilops longissima	7	V	Qi et al. [20]	VLQTLPTMRNMYVPPY	0
[GenBank: ACJ03516.1]	Aegilops searsii	7	IV	Qi et al. [20]	LAQIPQQLQYAAIHS	0
[GenBank: ACI04088.1]	T. aestivum cv. Jinan 177	7	V	Chen et al. [21]	EAIRSLVLQTLPSMSNVYVPP	0
[GenBank: AAK84776.1]	T. aestivum cv. Cheyenne	9	II	Anderson et al. [14]	HTFPQPQQTCPHQPQQQFPQ	0
[GenBank: ACJ03499.1]	T. urartu	9	II	Qi et al. [20]	PFCQHQQPFYQQPQQTFPHQPQ	0
[GenBank: CAI78902.1]	T. aestivum cv. Neepawa	9	II	Snegaroff, unpublished	PFPQSQQQCLQQPQHQFPQPTQQ	0
[GenBank: ACI04102.1]	T. aestivum × Lophopyrum elongatum	9	V	Chen et al. [21]	RHTDPATTVSACPGQGIIQPQQ	3
[GenBank: ACI04105.1]	T. aestivum × Lophopyrum elongatum	9	V	Chen et al. [21]	RHTDPATTVSACPGQGIIQPQQ	3
[GenBank: ACI04108.1]	T. aestivum × Lophopyrum elongatum	9	II	Chen et al. [21]	PFPQPQQPFCQQPQQPYP	0

¹ The extra cysteine in proteins containing nine cysteines or residue substituted for cysteine in proteins containing seven cysteines is underlined.
² determined by tBLASTn search of non-human, non-mouse ESTs, limited to Triticum, word size 2, expect 30000, PAM30, no compositional adjustment, no filters, no masks, database last searched on 7/22/09.

ISSN: 1471-2229