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Table 3 Comparison of gamma gliadins encoded by Butte 86 contigs to proteins in NCBI and proteins encoded by contigs from other EST assemblies.

From: Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour

Butte 86 Contig # # Amino acids NCBI Accession #1 Identity TaGI 10.0 Contig # Identity TaGI 11.0 Contig # Identity US Wheat Genome Project Contig # Identity2 HarvEST 1.14 Contig # Identity2
1 328 [GenBank: AAK84774.1] 326/337 TC2347103 194/197 TC2918573 181/193 185244 nd 14880 328/328
     TC250086 328/328 TC280138 327/328    19014,5 nd
     TC250043 326/337       
2 327 [GenBank: AAK84779.1] 326/327 TC250310 327/327 TC327998 327/327 188714 nd 4446 327/327
       TC3330203 160/198    149393,4 nd
3 302 [GenBank: AAA34272.1] 300/302 TC249992 300/302 TC308951 299/302 18764 299/302 2079 302/302
4 302 [GenBank: ACJ03466.1] 302/302 TC250000 302/302 TC308951 276/302 18752 301/302 149393,4 nd
       TC337354 269/302    149453 212/230
5 358 [GenBank: ACJ03521.1] 342/362 TC233870 357/358 TC3573196 357/358 188737 nd 15356 358/358
     TC250310 306/358       
6 285 [GenBank: ABO37962] 283/285 TC249991 285/285 TC3299763 254/264 18814 285/285 15101 285/285
       TC332920 265/285     
7 291 [GenBank: ACJ03428.1] 290/291 TC232926 291/291 TC296897 290/291 18753 289/291 4448 290/291
           19008 291/291
8 332 [GenBank: AAK84774.1] 317/337 TC250043 317/337 TC280138 313/332 185244 nd 14880 314/332
     TC2347103 194/197 TC281237 318/339 2713 176/176 46049 nd
     TC250086 314/332 TC2918573 183/185    19014,5 nd
           474323 188/188
93 289 [GenBank: ACJ03517.1] 266/294 TC2499963 204/227 TC293526 283/289 16926 288/289 14942 288/289
     TC2500313 251/255       
10 286 [GenBank: AAA34272.1] 284/302 TC249992 284/302 TC308951 283/302 18764 283/302 2079 286/302
       TC3377603 142/180 188424 nd 2095 283/286
113 163 [GenBank: ACJ03465.1] 163/163 TC233421 163/163 TC277980 163/163 165853 163/163 4328 163/163
  1. 1 accession with highest score in BLASTp search of non-redundant protein sequences using BLOSUM62, no compositional adjustments, no filters, no masks. Database last searched on 5/08/09.
  2. 2 protein identities that were not determined because consensus sequences of contigs contained frameshifts or stop codons in the reading frame are indicated by nd.
  3. 3 partial coding sequence.
  4. 4 consensus sequence contains frameshift or stop codon in reading frame.
  5. 5 complement of consensus sequence.
  6. 6 contig contains a portion of a LMW-GS sequence at 5' end.
  7. 7 encodes alpha gliadin.
  8. 8 encodes identical protein to Butte 86 contig, but does not contain any ESTs from Butte 86.
  9. 9 encodes LMW-glutenin subunit.