Figure 4
Effect of inhibition of cellulase activity on the induction of alamethicin resistance of tobacco cells. Resistance was measured as per cent of respiration rate remaining after 10 min incubation with 20 µg ml-1 alamethicin compared to the initial rate. Samples were pre-incubated with combinations of 1% cellulase, 0.1% macerozyme, 0.1 M glucose, and 0.1 M cellobiose in 0.35 M mannitol for 20 min followed by 220 min with control medium only. Where glucose or cellobiose was included, the concentration of mannitol in the control medium was reduced to give a similar molarity. M, control cells, CM, CM-treated cells, G, glucose, C, cellobiose. Data shown are averages of two biological replicates and error bars represent SD. Student's t-test was performed relative to the CM sample with * denoting p< 0.05 and *** denoting p < 0.001.