Schematic representation of the predicted miRNA395-ATP sulphurylase mRNA interaction in an IL population ( Solanum lycopersicum × Solanum pennellii ). The top panel shows the genetic map position of miRNA395a/b/c loci in the long arm of tomato chromosome 5 (BIN B) and the physical contig of the three miRNA precursors on the BAC clone (C05HBz0058L13 GenBank Acc # AC194694). Black arrows indicate the direction of precursor's transcription and the cluster size is also indicated. The middle panel shows the predicted secondary structures of the three pre-miRNA395 (a/b/c) from the two parental lines and also from the introgressed lines 5-1. Gray lines on the right of each stem-loop indicate the regions coding the mature miRNAs. The bottom panel shows the alignments between the miRNA395a/b/c and their target gene ATP-sulphurylase mRNA. Arrows indicate polymorphism on the miRNA395c (black: substitutions and white deletion).