Analysis of promoter activity of members of the PECTATE LYASE-LIKE (PLL) gene family in cell separation in Arabidopsis
© Sun and van Nocker; licensee BioMed Central Ltd. 2010
Received: 2 March 2010
Accepted: 22 July 2010
Published: 22 July 2010
Pectate lyases depolymerize pectins by catalyzing the eliminative cleavage of α-1,4-linked galacturonic acid. Pectate lyase-like (PLL) genes make up among the largest and most complex families in plants, but their cellular and organismal roles have not been well characterized, and the activity of these genes has been assessed only at the level of entire organs or plant parts, potentially obscuring important sub-organ or cell-type-specific activities. As a first step to understand the potential functional diversity of PLL genes in plants and specificity of individual genes, we utilized a reporter gene approach to document the spatial and temporal promoter activity for 23 of the 26 members of the Arabidopsis thaliana (Arabidopsis) PLL gene family throughout development, focusing on processes involving cell separation.
Numerous PLL promoters directed activity in localized domains programmed for cell separation, such as the abscission zones of the sepal, petal, stamen, and seed, as well as the fruit dehiscence zone. Several drove activity in cell types expected to facilitate separation, including the style and root endodermal and cortical layers during lateral root emergence. However, PLL promoters were active in domains not obviously programmed for separation, including the stipule, hydathode and root axis. Nearly all PLL promoters showed extensive overlap of activity in most of the regions analyzed.
Our results document potential for involvement of PLL genes in numerous aspects of growth and development both dependent and independent of cell separation. Although the complexity of the PLL gene family allows for enormous potential for gene specialization through spatial or temporal regulation, the high degree of overlap of activity among the PLL promoters suggests extensive redundancy. Alternatively, functional specialization might be determined at the post-transcriptional or protein level.
Pectins, a class of polysaccharide polymer typically characterized by a linear backbone of α-1,4-linked galacturonic acid (GalA) residues, are a major component of primary plant cell walls, and within the wall form a matrix in which a network of cellulose and hemicellulose is embedded [1–3]. Pectins contribute to the mechanical strength and physical properties of primary cell walls, but also function in intercellular adhesion [4–6], and can act as signaling molecules in morphogenesis and pathogen defense .
Plant growth and development is accompanied by dynamic remodeling of the cell wall, which in turn requires modifications of the various cell wall components including pectin. In accordance with the often complex structure of some pectins, an assortment of pectinase activities modify or degrade these polymers. Pectinesterases target methyl-esterified homogalacturonan, yielding substrates for polygalacturonases and pectate lyases, which cleave the GalA backbone . Rhamnogalacturonases and rhamnogalacturonan lyases depolymerize branched regions of rhamnogalacturonan, whereas β-galactosidases and α-arabinosidases can degrade the galactan/arabinan/arabinogalactan side chains. Pectate lyases (EC 126.96.36.199) have been most extensively studied in Erwinia chrysanthemi, a major causal agent of soft-rot diseases that affect a wide range of plant species [7–9]. Their action not only results in maceration of plant tissues but also can activate plant defense systems [10–12]. Plant PECTATE LYASE-LIKE (PLL) genes encode proteins with strong amino acid sequence homology with the PelC isoform of bacterial pectate lyases [13, 14] and exist as large families in plants where studied . The abundance of PLL genes in plants, including 26 in Arabidopsis, 12 in rice, and 22 in poplar, has arisen from multiple gene duplication events [16, 17], a process that may enhance plasticity in adaptation to changing environments . Models predict that gene redundancy is evolutionarily stable only when duplicated genes differentiate in some aspect of their function, suggesting that individual members of large families such as PLL may have some unique function .
Various analyses of PLL genes from plants revealed expression in a broad range of organs and plant parts including root, leaf, flower, pollen, filament, style, pistil, and ripening fruit [14, 16, 19–24]. PLL genes in several studies also showed elevated expression in response to auxin, wounding, and/or pathogen infection [16, 25–28]. Transcriptional analysis in Arabidopsis revealed that a small subset of PLL genes were up-regulated during stamen abscission or in cortical cell separation during the emergence of the lateral root [16, 29, 30]. Reduced expression of strawberry PL1 in transgenic strawberry plants suggested a natural role in tissue softening during fruit ripening . All of these data implicate PLL genes in various plant growth and development events. However, the activity of these genes has been characterized only at the level of entire organs or plant parts, potentially obscuring important sub-organ or cell-type-specific activities.
During plant growth and development, there are many events in which adjacent cells separate in a coordinated manner. Cell separation resulting in organ abscission, or anther or fruit dehiscence, occurs in predetermined positions, called abscission zones (AZs) or dehiscence zones (DZs), respectively [32, 33]. Intercellular space formed in leaves and stems can result from restricted separation of cells at the tricellular regions . Fruit ripening also involves limited cell separation, in which only the middle lamella is degraded, with tricellular junctions and plasmodesmata often remaining intact [35, 36]. Targeted cell separation is also involved in the processes of seed germination, lateral root emergence, pollen tube penetration through the transmitting tract, and shedding of columella root cap cells [37, 38]. In this study, we examined spatial-temporal expression patterns of promoters of PLL family members, focusing on the various cell separation and wall loosening events that occur during Arabidopsis growth and development.
Results and Discussion
Estimation of PLL gene expression through analysis of public transcriptome data
As a first step to assess expression pattern of PLL genes in Arabidopsis, we analyzed publicly available transcriptome data. Expressed sequence tags (ESTs) found in public databanks corresponding to individual PLL gene family members were found to be sourced from various tissues and stages across Arabidopsis growth and development, as well as various environmental conditions (not shown). Representational frequency of ESTs for individual PLL genes was highly variable both across the gene family and within subfamilies (Figure 1). Analysis of publicly available microarray data also suggested that there was at least some unique developmental pattern of expression for most PLL genes (see Additional file 1). A striking exception are the four members of Subfamily II (PLL8-PLL11), which showed a similar expression pattern localized predominately to pollen and stamens (see Additional file 1). Several genes (PLL3, 4, 6, 7, 12, and 24) were transcriptionally silenced across most sampled tissues, whereas PLL2 was apparently expressed ubiquitously, suggesting a very general function (see Additional file 1). These data are generally consistent with the results of qualitative RT-PCR analysis of PLL genes  and support a collectively ubiquitous function for PLLs in growth and development and the potential for functional specialization by many of these genes.
Analysis of PLL promoter activity
Direct approaches for analysis of gene expression such as RNA-seq, microarray analysis and RT-PCR can be problematic when applied to large gene families, due to sequence homology among family members and/or inability to resolve expression at the cellular level. Accordingly, we utilized a histological reporter gene (β-glucuronidase, GUS) to determine the expression regulatory potential of individual PLL gene promoters. We engineered the GUS coding sequence adjacent to up to ~2 kb of 5' UTR/promoter sequence, preserving the authentic start codon of the PLL genes, and expressed the PLL::GUS fusions in transgenic Arabidopsis (see Additional file 2). For each PLL gene, we analyzed at least four independent transgenic lines. GUS activity patterns were generally consistent between independent lines, with the exception of three (PLL6, PLL12, and PLL14) that showed weak and variable activity and were not analyzed further. No GUS activity was observed in transgenic plants transformed with a promoterless GUS construction, or in non-transgenic plants, in any plant part or under any condition used for analysis. Based on the known roles for pectins in cell adhesion and cell wall architecture, we focused our analysis on developmental events closely associated with cell separation.
PLL::GUS expression associated with cell separation
Fruit DZ and seed AZ
A mostly overlapping subset of 16 PLL promoters drove GUS activity within the apparent seed AZ (Figure 3B and see Additional file 3). For all of these, GUS activity was not observed at the beginning of stage 19, but was obvious in seeds that were still attached to the funiculus and septum before the beginning of Stage 20 (e.g., PLL19 in Figure 3B).
Radicle emergence during seed germination
Lateral root initiation
Arabidopsis lateral roots initiate from the pericycle cell layer and must penetrate the overlaying endodermal, cortical and epidermal layers during emergence . Although several PLL promoters drove GUS activity at relatively weak levels along the axis of primary roots (see below), six (PLL16, 19, 21-23, 26) showed strong activity in primary roots of 12-d-old plants that were initiating lateral roots. This activity was confined to the endodermal and cortical layers in the region of initiation, but was not obviously restricted to cell layers directly overlaying the new lateral root primordia (Figure 4B and not shown). We did not observe strong GUS staining in the epidermis for any of the six promoters (Figure 4B and not shown). These findings are consistent with the increased transcriptional accumulation previously reported for PLL16 and PLL26 during lateral root emergence induced by auxin [26, 30].
PLL::GUS expression in other developmental contexts
To analyze PLL promoter activity in a broader developmental context, including processes potentially independent of cell separation, we undertook a comprehensive analysis of the activity of all of the studied PLL promoters during growth and development of Arabidopsis.
Hydathodes and Stipules
GUS activity was apparent within the shoot apex of plants in the vegetative phase for 19 PLL promoters. Upon close examination, in all cases this activity appeared in tightly localized foci bordering the base of initiating leaves, including cauline leaves (Figure 6B and not shown), at the position of the stipules. In Arabidopsis, stipules are apparent on the newly formed leaves but become less obvious upon leaf expansion. For all PLLs, GUS activity was strongest in those leaves that had initiated and were undergoing expansion, and weakest or not apparent in fully expanded leaves (Figure 6C and not shown). The function of stipules in Arabidopsis development remains largely unknown. However, it has been demonstrated that stipules are primary sites for the accumulation of auxin associated with vascular differentiation and leaf morphogenesis [45, 46]. No GUS activity was observed for any PLL promoter in the apex after the transition to flowering (not shown).
Base of flower/fruit pedicel
Correlation or divergence of PLL promoter activity with patterns of RNA accumulation
To evaluate the potential for regulation of PLL genes outside of the context of promoter activity, we analyzed our findings in relation to previously reported transcriptional patterns based on microarray analysis of Arabidopsis organs or whole structures (see above and Additional file 1 .
Most notably, the strong promoter activity found in anthers for the four members of Subfamily II (PLL8-PLL11) (Figure 8) corresponded well with the stamen-associated pattern of mRNA localization (see Additional file 1). However, PLL8, 9, and 11 also showed strong promoter activity variously outside of flowers in the hypocotyl (PLL11), root/hypocotyl junction (PLL8 and 9), and stipules (PLL8 and 11), regions where transcriptional profiling did not obviously predict promoter activity. Genes expected to be transcriptionally quiescent based on microarray analysis, such as PLL4 or PLL24, showed promoter activity that was relatively strong, and that was similar to genes that showed strong transcriptional signals such as PLL8 or PLL15, respectively. PLL2, which might be expected to be constitutively expressed as a 'housekeeping' gene based on microarray analysis, showed promoter activity in only a subset of the regions analyzed (see Additional file 3). At least part of these apparent inconsistencies between promoter activity analysis and transcriptional profiling can be attributed to our qualitative representation of promoter activity, which is in reality a quantitative function. The lack of high values for mRNA accumulation in structures (e.g., the silique) that include tissues with strong promoter activity (e.g., the DZ) could be simply explained by the fact that such tissues make up only a small portion of the bulk of the regions subject to analysis. Other cases where mRNA levels are low in spite of strong regional promoter activity might be attributed to rapid mRNA turnover. It is most difficult to explain the accumulation of mRNAs in tissues or structures that lack detectable promoter activity, for example the strong and ubiquitous mRNA expression of PLL2. In these cases regulatory elements required for appropriate expression might lay outside of the ~2 kb 5' region utilized to direct GUS expression.
In this study we determined the temporal and regional promoter activity for members of the PLL gene family during growth and development of Arabidopsis. Given the ubiquitous role of pectins in intercellular adhesion and cell wall architecture, and the various organismal processes that require targeted cell separation or cell wall remodeling, these genes are expected to be of fundamental importance.
We found that, as expected, promoter activity was common in cell types programmed for abscission, most obviously the perianth floral organ AZs, the DZ of the fruit, and the seed AZ. Activity was also seen in regions, such as cell layers in the region of initiating lateral roots, where cell separation is expected but has not been well characterized. Pectate lyase activity is expected to act in concert with other cell wall remodeling activities both to condition cell separation and to modify newly exposed cells for barrier functions. Interestingly, we saw more limited PLL promoter activity at the base of pedicels of flowers and fruits. Cells in these regions can be artificially programmed to undergo separation in Arabidopsis, in response to ectopic activity of the small abscission-associated peptide IDA , suggesting an abscission-related role. If these PLL genes do have a cryptic function in flower/fruit abscission, abscission may be normally limited by the degree of pectate lyase activity or deficiency of other, cooperative, cell wall-modifying activities. Interestingly, the two PLL promoters driving activity in this region also were active in cells at the base of trichomes, suggesting their pedicel- and trichome-associated roles may be analogous. Both regions are subject to mechanical stress imparted by movement of the pedicels/trichomes and an alternative function for these PLLs may be to participate in a pathway of cell wall remodeling to accommodate such stress.
Other patterns of PLL activity were difficult to explain given a function limited to cell separation, and might instead be associated with general cell wall remodeling accompanying growth and differentiation. The extensive and various activities seen in the root is a good example of this. We can not provide a simple explanation for the strong and widespread promoter activity in stipules; however, these structures are absorbed into the leaf periphery during leaf expansion and this process could be accompanied by radical changes in cell wall morphology. Interestingly, both stipules and hydathodes were previously characterized as sites of high free auxin concentration during early leaf development . A subset of PLL promoters in our study were found to be actively associated with lateral root emergence, or in the region of the root apical meristem, other sites associated with local auxin concentration maxima [30, 50]. These observations suggest a close functional relationship between these PLLs and auxin.
We note that many promoter activities of the PLL genes are superficially similar to those exhibited collectively by several presumed polygalacturonase genes . These include cases intuitively related to cell separation (perianth organ AZs, sites of lateral root emergence, fruit DZ, seed AZ, germinating seed) and cases in which activity may be limited to cell wall remodeling (developing pollen and seeds). This implies close functional association between PLLs and PGs, which is expected given that both should target pectins.
An obvious use of data from high-throughput analyses of promoter activity and/or transcriptional profiles is to guide reversed-genetics analyses, especially where phenotypes are difficult to anticipate and/or where potential redundancy of the targeted genes is a limitation. The original intent of this project was to identify individual or small subsets of PLL genes that might be targeted in this manner. However, the surprisingly high degree of overlap both in promoter activity and RNA accumulation observed among the PLL genes/promoters largely precluded this objective. We note that neither our promoter activity analysis nor previous microarray data would intuitively predict the documented non-redundant role for PLL13 (PMR6) in facilitating infection by powdery mildew . This role is accomplished where infection occurs in leaf cells, where neither transcriptional profiling nor our promoter analysis suggested strong or non-redundant expression.
Another obvious application of transcriptional data is the computational identification of DNA regulatory elements linked to regionally or temporally specific expression patterns. Here, it is noteworthy that the correlation between the transcriptional profiles previously established for these genes [16, 48] and their promoter activity (this study) was apparently very limited. This justifies the additional time and expense necessary to accomplish comprehensive promoter activity profiles, and/or cell-type specific transcriptional profiling to define transcriptional regulatory elements.
The PLL gene family is one of the largest and most complex in Arabidopsis, suggesting enormous potential for gene specialization through spatial or temporal regulation. However, we found a remarkably high degree of overlap of activity among the PLL promoters. The activity of 19 of the 23 tested PLLs in the stipules is an excellent example of this, as is the activity of at least 18 in the apparent AZs of the sepals, petals and stamens at similar stages of development. This observation is surprising given that gene duplications should be evolutionarily stable only after having acquired a useful, non-redundant function. For PLLs, functional specificity may be bestowed by post-transcriptional and downstream mechanisms. Such specificity might include regulation of RNA stability. Specificity might also be directed at the protein level, through potential differences in localization or mobility within the apoplast. Alternatively, given the expected dynamic pH environment of the apoplast, the proteins might function only within a narrow pH window. Finally, various PLL proteins might show preference for specific pectin substrates, an intriguing possibility given the structural complexity of pectins. Many of these possibilities could be addressed by the purification (or recombinant synthesis) and enzymatic characterization of individual PLLs.
Plant growth conditions
Wild type Arabidopsis thaliana (ecotype Col-0) was used for all experiments. Standard growth conditions were 16-h-cool white fluorescent light/8-h-dark photoperiods at 22°C. For growth in sterile culture, seeds were surface-sterilized and germinated on one-half-strength Murashige and Skoog (MS) medium supplemented with 10 mM MES [2-(N-morpholino) ethanesulfonic acid] (Sigma, St. Louis, MO), pH adjusted to 5.7, and solidified with 0.8% phytoagar (Invitrogen, Carlsbad, CA).
Phylogenetic analysis and protein domain identification
The peptide sequence of an enzymatically confirmed pectate lyase from banana, Musa acuminata PL1 (GenBank accession AF206319) , was utilized as a query in BLASTP (Basic Local Alignment Search Tool Protein) analysis of Arabidopsis translated open reading frames [The Arabidopsis Information Resource (TAIR) Version 7] , and closely related proteins [Expect (E) value < 8E-36] were used for phylogenetic tree construction. Predicted amino acid sequences were aligned using ClustalW2 [53, 54], and MEGA4  was used to construct the phylogenetic tree. Annotated PLL protein domains were identified in the Pfam database [56, 57] as follows: PF03211, Pectate_lyase; PF00544, Pec_lyase_C; PF09492, Pec_lyase; PF04431, Pec_lyase_N. Amino-terminal signal peptides were defined by SignalP 3.0  using a maximum S-score larger than 0.95 and signal peptide probability greater than 0.80 . The number of ESTs for each gene in the NCBI Unigene database was found at NCBI .
Transgenic plant construction
To engineer GUS as a reporter for PLL promoter activity, up to approximately 2 kb of genomic DNA, upstream from the start codon, was amplified by PCR using gene-specific primers (see Additional file 4) containing unique restriction sites. In general, where the upstream intergenic region was less than 2 kb, all of the region was included (see Additional file 2). All amplification products were sequenced to confirm lack of PCR-induced mutation, and cloned into the pCAMBIA1305 vector (Cambia, Black Mountain, Australia) modified to contain an NcoI site at the start codon of the GUS gene and multiple cloning sites replacing the pCAMBIA Lac Z alpha and CaMV35S regions. This resulted in PLL::GUS fusions that preserve the authentic 5' UTR and start codon position. Plasmid DNAs were introduced into Agrobacterium tumefaciens strain GV3101 and transformed into wild-type plants using the floral dipping method . Transgenic plants were subjected to selection with the herbicide glufosinate (Basta; Beyer CropScience, Barmen, Germany).
Histochemical GUS assays
Populations derived from at least four independently transformed transgenic lines were analyzed for each PLL::GUS construction. For histochemical GUS assays, plants or plant parts at various developmental stages were immersed in GUS staining solution [0.5 mM X-gluc, 0.5% (v/v) Triton X-100, 50 mM sodium phosphate buffer (pH adjusted to 7.2)] under vacuum infiltration for 5 min, and incubated at 37°C for various lengths of time. After staining, tissues were incubated in 70% ethanol for several hours to remove chlorophyll. Staining was visualized using a Nikon dissecting microscope equipped with a digital camera. Flower developmental stages annotated in this study were described by Smyth et al .
Analysis of AtGenExpress data
Microarray data were collected from the AtGenExpress Development data set . Only results from wild-type plants were included in the analysis. The log2-transformed absolute signal values were used in hierarchical clustering based on average linkage (Cluster version 3; ), and results were visualized in TreeView .
This work was funded by grants from the Michigan Agricultural Experiment Station (MAES) and Washington Tree Fruit Research Commission (WTFRC) to SvN, and by funding from the USDA Multistate Research Project, NE1018, "Postharvest Biology of Fruit" to LS. We thank Dr. Art Cameron (Michigan State University) for assistance with microscopy and photography.
- Caffall KH, Mohnen D: The structure, function, and biosynthesis of plant cell wall pectic polysaccharides. Carbohydr Res. 2009, 344: 1879-1900. 10.1016/j.carres.2009.05.021.PubMedView ArticleGoogle Scholar
- Ridley BL, O'Neill MA, Mohnen D: Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry. 2001, 57: 929-967. 10.1016/S0031-9422(01)00113-3.PubMedView ArticleGoogle Scholar
- Willats WG, McCarney L, Mackie W, Knox PJ: Pectin: cell biology and prospects for functional analysis. Plant Mol Biol. 2001, 47: 9-27. 10.1023/A:1010662911148.PubMedView ArticleGoogle Scholar
- Iwai H, Masaoka N, Ishii T, Satoh S: A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem. Proc Natl Acad Sci USA. 2002, 99: 16319-16324. 10.1073/pnas.252530499.PubMedPubMed CentralView ArticleGoogle Scholar
- Jarvis M, Briggs S, Knox J: Intercellular adhesion and cell separation in plants. Plant Cell Enviro. 2003, 26: 977-989. 10.1046/j.1365-3040.2003.01034.x.View ArticleGoogle Scholar
- Knox JP: Cell and developmental biology of pectins. Pectins and Their Manipulation. Edited by: Seymour GB, Knox JP. 2002, CRC press LLC, 131-146.Google Scholar
- Barras F, van Gijsegem F, Chatterjee AK: Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu Rev Phyto. 1994, 32: 201-234.View ArticleGoogle Scholar
- Collmer A, Keen N: The role of pectic enzymes in plant pathogenesis. Annu Rev Phytopathol. 1986, 24: 383-409. 10.1146/annurev.py.24.090186.002123.View ArticleGoogle Scholar
- Kotoujansky A: Molecular genetics of pathogenesis by soft rot Erwinias. Annu Rev Phytopathol. 1987, 25: 405-430. 10.1146/annurev.py.25.090187.002201.View ArticleGoogle Scholar
- De Lorenzo G, Cervone F, Hahn MG, Darvill A, Albersheim P: Bacterial endopectate lyase: evidence that plant cell wall pH prevents tissue maceration and increases the half-life of elicitor-active oligogalacturonides. Physiol Mol Plant Pathol. 1991, 39: 335-344. 10.1016/0885-5765(91)90015-A.View ArticleGoogle Scholar
- Fagard M, Dellagi A, Roux C, Périno C, Rigault M, Boucher V, Shevchik V, Expert D: Arabidopsis thaliana expresses multiple lines of defense to counterattack Erwinia chrysanthemi. Mol Plant Microbe Interact. 2007, 20: 794-805. 10.1094/MPMI-20-7-0794.PubMedView ArticleGoogle Scholar
- Norman-Setterblad C, Vidal S, Palva ET: Interacting signal pathways control defense gene expression in A. thaliana in response to cell wall-degrading enzymes from Erwinia carotovora. Mol Plant Microbe Interact. 2000, 13: 430-438. 10.1094/MPMI.2000.13.4.430.PubMedView ArticleGoogle Scholar
- Wing R, Yamaguchi J, Larabell S, Ursin V, McCormic S: Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogen Erwinia. Plant Mol Biol. 1990, 14: 17-28. 10.1007/BF00015651.PubMedView ArticleGoogle Scholar
- Marín-Rodríguez MC, Orchard J, Seymour GB: Pectate lyases, cell wall degradation and fruit softening. J Exp Bot. 2002, 53: 2115-2119. 10.1093/jxb/erf089.PubMedView ArticleGoogle Scholar
- Henrissat B, Coutinho PM, Davies GJ: A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana. Plant Mol Biol. 2001, 47: 55-72. 10.1023/A:1010667012056.PubMedView ArticleGoogle Scholar
- Palusa SG, Golovkin M, Shin SB, Richardson DN, Reddy AS: Organ-specific, developmental, hormonal and stress regulation of expression of putative pectate lyase genes in Arabidopsis. New Phytol. 2007, 174: 537-550. 10.1111/j.1469-8137.2007.02033.x.PubMedView ArticleGoogle Scholar
- Zhang Z: Evolution by gene duplication: an update. Trends Ecol Evol. 2003, 18: 292-298. 10.1016/S0169-5347(03)00033-8.View ArticleGoogle Scholar
- Lynch M, Force A: The probability of duplicate gene preservation by subfunctionalization. Genetics. 2000, 154: 459-473.PubMedPubMed CentralGoogle Scholar
- Benitéz-Burraco A, Blanco-Portales R, Redondo-Nevado J, Bellido ML, Moyano E, Caballero JL, Muñoz-Blanco J: Cloning and characterization of two ripening-related strawberry (Fragaria × ananassa cv. Chandler) pectate lyase genes. J Exp Bot. 2003, 54: 633-645. 10.1093/jxb/erg065.PubMedView ArticleGoogle Scholar
- Chourasia A, Sane VA, Nath P: Differential expression of pectate lyase during ethylene-induced postharvest softening of mango (Mangifera indica var. Dashehari). Physiol Plant. 2006, 128: 546-555. 10.1111/j.1399-3054.2006.00752.x.View ArticleGoogle Scholar
- Futamura N, Kouchi H, Shinohara K: A gene for pectate lyase expressed in elongating and differentiating tissues of a Japanese willow (Salix gilgiana). J Plant Physiol. 2002, 159: 1123-1130. 10.1078/0176-1617-00837.View ArticleGoogle Scholar
- Marín-Rodríguez M, Smith D, Manning K, Orchard J, Seymour GB: Pectate lyase gene expression and enzyme activity in ripening banana fruit. Plant Mol Biol. 2003, 51: 851-857. 10.1023/A:1023057202847.PubMedView ArticleGoogle Scholar
- Mita S, Nagai Y, Asai T: Isolation of cDNA clones corresponding to genes differentially expressed in pericarp of mume (Prunus mume) in response to ripening, ethylene and wounding signals. Physiol Plant. 2006, 128: 531-545. 10.1111/j.1399-3054.2006.00749.x.View ArticleGoogle Scholar
- Pua E, Ong C, Liu P, Liu J: Isolation and expression of two pectate lyase genes during fruit ripening of banana (Musa acuminata). Physiol Plant. 2001, 113: 92-99. 10.1034/j.1399-3054.2001.1130113.x.View ArticleGoogle Scholar
- Domingo C, Roberts K, Stacey NJ, Connerton I, Ruiz-Teran F, McCann M: A pectate lyase from Zinnia elegans is auxin inducible. Plant J. 1998, 13: 17-28. 10.1046/j.1365-313X.1998.00002.x.PubMedView ArticleGoogle Scholar
- Laskowski M, Biller S, Stanley K, Kajstura T, Prusty R: Expression profiling of auxin-treated Arabidopsis roots: toward a molecular analysis of lateral root emergence. Plant Cell Physiol. 2006, 47: 788-792. 10.1093/pcp/pcj043.PubMedView ArticleGoogle Scholar
- Milioni D, Sado PE, Stacey NJ, Domingo C, Roberts K, McCann MC: Differential expression of cell-wall-related genes during the formation of tracheary elements in the Zinnia mesophyll cell system. Plant Mol Biol. 2001, 47: 221-238. 10.1023/A:1010647902487.PubMedView ArticleGoogle Scholar
- Vogel JP, Raab T, Somerville C: PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis. Plant Cell. 2002, 14: 2095-2106. 10.1105/tpc.003509.PubMedPubMed CentralView ArticleGoogle Scholar
- Cai S, Lashbrook CC: Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2. Plant Physiol. 2008, 146: 1305-1321. 10.1104/pp.107.110908.PubMedPubMed CentralView ArticleGoogle Scholar
- Swarup K, Benková E, Swarup R, Casimiro I, Péret B, Yang Y, Parry G, Nielsen E, Smet ID, Vanneste S, Levesque MP, Carrier D, James N, Calvo V, Ljung K, Kramer E, Roberts R, Graham N, Marillonnet S, Patel K, Jones JDG, Taylor CG, Schachtman DP, Sandberg G, Benfey P, Friml J, Kerr I, Beeckman T, Laplaze L, Bennett MJ: The auxin influx carrier LAX3 promotes lateral root emergence. Nat Cell Biol. 2008, 10: 946-954. 10.1038/ncb1754.PubMedView ArticleGoogle Scholar
- Jiménez-Bermúdez S, Redondo-Nevado J, Muñoz-Blanco J, Caballero JL, López-Aranda JM, Valpuesta V, Pliego-Alfaro F, Quesada MA, Mercado JA: Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene. Plant Physiol. 2002, 128: 751-759. 10.1104/pp.010671.PubMedPubMed CentralView ArticleGoogle Scholar
- Leslie ME, Lewis MW, Liljegren SJ: Organ abscission. Annual Plant Reviews. Edited by: Roberts JA, Gonzalez-Carranza Z. 2007, Oxford: Blackwell Publishing Ltd, 25: 91-106.Google Scholar
- Ostergaard L, Borkhardt B, Ulvskov P: Dehiscence. Annual Plant Reviews. Edited by: Roberts JA, Gonzalez-Carranza Z. 2007, Oxford: Blackwell Publishing Ltd, 25: 137-159.Google Scholar
- Jarvis M: Intercellular separation forces generated by intracellular pressure. Plant Cell Enviro. 1998, 21: 1307-1310. 10.1046/j.1365-3040.1998.00363.x.View ArticleGoogle Scholar
- Roy S, Jauneau A, Vian B: Analytical detection of calcium-ions and immunocytochemical visualization of homogalacturonic sequences in the ripe cherry tomato. Plant Physiol Biochem. 1994, 32: 633-640.Google Scholar
- Hallett IC, MacRae EA, Wegrzyn TF: Changes in kiwifruit cell-wall ultrastructure and cell packing during postharvest ripening. Int J Plant Sci. 1992, 153: 49-60. 10.1086/297006.View ArticleGoogle Scholar
- Wen F, Laskowski M, Hawes M: Cell separation in roots. Annual Plant Reviews. Edited by: Roberts JA, Gonzalez-Carranza Z. 2007, Oxford: Blackwell Publishing Ltd, 25: 91-106.Google Scholar
- Mollet JC, Faugeron C, Morvan H: Cell adhesion, separation and guidance in compatible plant reproduction. Annual Plant Reviews. Edited by: Roberts JA, Gonzalez-Carranza Z. 2007, Oxford: Blackwell Publishing Ltd, 25: 69-90.Google Scholar
- Patterson SE: Cutting loose. Abscission and dehiscence in Arabidopsis. Plant Physiol. 2001, 126: 494-500. 10.1104/pp.126.2.494.PubMedPubMed CentralView ArticleGoogle Scholar
- Smyth DR, Bowman JL, Meyerowitz EM: Early flower development in Arabidopsis. Plant Cell. 1990, 2: 755-767. 10.1105/tpc.2.8.755.PubMedPubMed CentralView ArticleGoogle Scholar
- Ferrándiz C: Regulation of fruit dehiscence in Arabidopsis. J Exp Bot. 2002, 53: 2031-2038. 10.1093/jxb/erf082.PubMedView ArticleGoogle Scholar
- Liu PP, Koizuka N, Homrichhausen TM, Hewitt JR, Martin RC, Nonogaki H: Large-scale screening of Arabidopsis enhancer-trap lines for seed germination-associated genes. Plant J. 2005, 41: 936-944. 10.1111/j.1365-313X.2005.02347.x.PubMedView ArticleGoogle Scholar
- Malamy JE, Benfey PN: Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development. 1997, 124: 33-44.PubMedGoogle Scholar
- Esau K: Hydathodes. Anatomy of Seed Plants. 1977, New York: John Wiley & Sons, 205-206.Google Scholar
- Aloni R, Schwalm K, Langhans M, Ullrich CI: Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis. Planta. 2003, 216: 841-853.PubMedGoogle Scholar
- Cheng Y, Dai X, Zhao Y: Auxin synthesized by the YUCCA flavin monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis. Plant Cell. 2007, 19: 2430-2439. 10.1105/tpc.107.053009.PubMedPubMed CentralView ArticleGoogle Scholar
- Berger F: Endosperm: the crossroad of seed development. Curr Opin Plant Biol. 2003, 6: 42-50. 10.1016/S1369526602000043.PubMedView ArticleGoogle Scholar
- Schmid M, Davison TS, Henz SR, Pape UJ, Demar M, Vingron M, Schölkopf B, Weigel D, Lohmann JU: A gene expression map of Arabidopsis thaliana development. Nat Genet. 2005, 37: 501-506. 10.1038/ng1543.PubMedView ArticleGoogle Scholar
- Stenvik GE, Butenko MA, Urbanowicz BR, Rose JK, Aalen RB: Overexpression of INFLORESCENCE DEFICIENT IN ABSCISSION activates cell separation in vestigial abscission zones in Arabidopsis. Plant Cell. 2006, 18: 1467-1476. 10.1105/tpc.106.042036.PubMedPubMed CentralView ArticleGoogle Scholar
- Sabatini S, Beis D, Wokenfelt H, Murfett J, Guilfoyle T, Malamy H, Benfey P, Leyser O, Bechtold N, Weisbeek P, Scheres B: An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root. Cell. 1999, 99: 463-472. 10.1016/S0092-8674(00)81535-4.PubMedView ArticleGoogle Scholar
- González-Carranza ZH, Elliott KA, Roberts JA: Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana. J Exp Bot. 2007, 58: 3719-3730. 10.1093/jxb/erm222.PubMedView ArticleGoogle Scholar
- The Arabidopsis Information Resource. [http://www.arabidopsis.org]
- Clustal: Multiple Sequence Alignment. [http://www.clustal.org]
- Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and Clustal x version 2.0. Bioinformatics. 2007, 23: 2947-2948. 10.1093/bioinformatics/btm404.PubMedView ArticleGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.PubMedView ArticleGoogle Scholar
- Pfam Database. [http://pfam.sanger.ac.uk/]
- Finn RD, Mistry J, Tate J, Coggill P, Heger A, Pollington JE, Gavin OL, Gunasekaran P, Ceric G, Forslund K, Holm L, Sonnhammer EL, Eddy SR, Bateman A: The Pfam protein families database. Nucleic Acids Res. 2010, 38: D211-22. 10.1093/nar/gkp985.PubMedPubMed CentralView ArticleGoogle Scholar
- SignalP 3.0 Server. [http://www.cbs.dtu.dk/services/SignalP/]
- Emanuelsson O, Brunak S, von Heijne G, Nielsen H: Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc. 2007, 2: 953-971. 10.1038/nprot.2007.131.PubMedView ArticleGoogle Scholar
- National Center for Biotechnology Information. [http://www.ncbi.nlm.nih.gov]
- Clough SJ, Bent AF: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998, 16: 735-743. 10.1046/j.1365-313x.1998.00343.x.PubMedView ArticleGoogle Scholar
- Eisen MB, Spellman PT, Brown PO, Botstein D: Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA. 1998, 95: 14863-14868. 10.1073/pnas.95.25.14863.PubMedPubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.