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Table 2 Kinetic constants for recombinant Arabidopsis PGGT1*

From: The CaaX specificities of Arabidopsis protein prenyltransferases explain era1 and ggb phenotypes

PGGT1
substrate
Km (μM) Specific Activity
(pmol min-1 mg-1)
kcat (hr-1) kcat/Km
(μM-1 hr-1)
n
GFP-BD-CVIL 17.2 +/- 4.4 110 +/- 30 0.50 +/- 0.12 0.029 +/- 0.010 7
GFP-BD-CVII 19.0 +/-14.4 17 +/- 15 0.08 +/- 0.07 0.004 +/- 0.005 4
GFP-BD-CVIQ 3.5 +/- 0.6 1.7 +/- 0.2 0.008 +/- 0.001 0.002 +/- 0.001 8
GFP-BD-CVIM 5.8 +/- 1.0 2.6 +/- 0.3 0.012 +/- 0.001 0.002 +/- 0.001 8
  1. Standard errors are given.
  2. *PGGT1 assays were performed in the presence of [1-3H]geranylgeranyl diphosphate.