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Table 1 Kinetic constants for recombinant Arabidopsis PFT*

From: The CaaX specificities of Arabidopsis protein prenyltransferases explain era1 and ggb phenotypes

PFT
substrate
Km (μM) Specific Activity
(pmol min-1 mg-1)
kcat (hr-1) kcat/Km
(μM-1 hr-1)
n
GFP-BD-CVIM 5.4 +/- 0.6 5200 +/- 700 28.8 +/- 4.1 5.3 +/- 1.0 8
GFP-BD-CVIQ 5.5 +/- 0.9 8900 +/- 1900 49.1 +/- 10.5 8.9 +/- 2.4 8
GFP-BD-CVII 6.9 +/- 1.0 2300 +/- 700 12.8 +/- 3.7 1.9 +/- 0.6 8
GFP-BD-CVIL 7.0 +/- 1.5 670 +/- 140 3.7 +/- 0.8 0.5 +/- 0.2 8
  1. Standard errors are given.
  2. *PFT assays were performed in the presence of [1-3H]farnesyl diphosphate.