Fig. 3

CrPINMa transcript analysis in edited plants. (a) Electrophoresis of RT-PCR products. The DNA ladder (M), a wild-type plant (1#), and four P1 progeny (2#, 3#, 4#, 5#) were included and the expected bands were boxed. (b) Sequencing of RT-PCR products focusing on the edited site. The top one (1#) showed the original sequence, the underneath one (2#) and the third one (4#) displayed the same mutation, namely the P1a mutation. The bottom one (5#) displayed the P1a and P1b transcripts. (c) Sequencing focusing on the intron region. The same sequence was generated in 1#, 2#, 4# and 5# (from top to bottom). Two red triangles indicated intron positions