Fig. 2

Identification of the edited plants. (a) Electrophoresis of the HindIII-digested PCR products spanning the two gRNA binding sites. M, molecular marker. P1–P8, investigated individuals. (b) Sequencing of the same PCR products. Double peaks suggested that there were two different alleles. (c) Mutation characterization of the edited alleles. The wildtype indicated an in-frame coding region spanning the two PAM motifs, which were highlighted by underlines. The HindIII site was also underlined, and the cutting positions by the editing machine were indicated by two red triangles. The P8e allele contained a premature stop codon highlighted by boxing