Fig. 8
Expression analysis of OsSIP1, OsSIP2 with stress response marker genes OsNAC066 (oxidative) and OsbZIP50 (ER stress) under oxidative and ER stress treatments. Two-weeks-old rice (O. sativa L. cv. Nipponbare) seedlings were treated separately with 1% H2O2 or 2 mM DTT (dithiothreitol). Whole plants were collected for qRT-PCR analysis after 3, 6, and 9 h treatments. Values represent the mean ± SD of three biological replicates and three technical replicates, and the Y-axis represents the relative expression level normalized to OsACTIN. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, and ***P < 0.001) based on Student t-test compared to CK (0 h time point without treatment)