Fig. 7

Expression analysis of OsSIP1, OsSIP2 with temperature response marker genes OsCFB3 (cold) and OsHSP14.7 (heat) under extreme temperature stress treatment. Two-weeks-old rice seedlings (O. sativa L. cv. Nipponbare) were treated separately at 4 °C or 42 °C. Whole plants were collected for qRT-PCR analysis after 3, 6, and 9 h treatments. Values represent the mean ± SD of three biological replicates and three technical replicates, and the Y-axis represents the relative expression level normalized to OsACTIN. Asterisks indicate significant differences (**P < 0.01, and ***P < 0.001) based on Student t-test compared to CK (0 h time point without treatment)