Fig. 4

Antioxidant assay of wild tpe (WT) and NtWRKY65 overexpression lines. Activity of (a) CAT, (b) SOD, (c) POD, and the levels of (d) MDA and (e) leaf relative conductance analyzed after treatment using 0 mM NaCl and 150 mM NaCl. Leaf ROS accumulation was visualized by (f) DAB and (g) NBT staining. Content of (h) H₂O₂ and (i) O₂⁻ after 0 mM NaCl and 150 mM NaCl treatment. Columns and bars represent the means and standard errors of three seedlings, respectively. Significant differences between the WT and overexpression lines were determined using Student’s t-test, * P < 0.05, ** P < 0.01