Fig. 1

Cis-element and activity analysis of the NtWRKY65 promoter. (a) Cis-elements of the NtWRKY65 promoter. An 1800 bp fragment upstream of the start codon was analyzed. Core promoter elements such as the TATA-box and CAAT-box are not shown for clarity. (b) Activity analysis of the NtWRKY65 promoter. Transgenic tobacco seedlings containing NtWRKY65 promoter driven β-glucuronidase (GUS) recombinant stained after 0 mM and 150 mM NaCl treatment for two hours. (c) The GUS activity in transgenic tobacco containing NtWRKY65 promoter driven GUS recombinant after 0 mM or 150 mM NaCl treatment for two hours. Columns and bars represent the mean and standard error of three seedlings, respectively. Asterisks indicate a significant difference at P < 0.05 by the Student’s t-test. (d) qRT-PCR analysis of NtWRKY65 expression levels at different time intervals under 150 mM NaCl treatment. The relative expression was calculated by setting 0 h as 1