Fig. 1

Genomic features of the B3 genes in seven plant genomes. (A) The number of B3 subfamilies and their domain architectures in seven plant genomes. Different symbols located on the left represent the corresponding domains. The color of the bars indicates subfamilies. The dots next to B3-REM express one or more of the B3 domains. (B) The ratio and number of subfamilies per species. The bar colors present each subfamily. (C) Multiple sequence alignments and secondary structures of B3 domain sequences in seven plant genomes. The secondary structure positions are shown above the amino acid logo. The height of the logos within the stack shows the relative frequency at each position. In the sequence alignment, it is capitalized if more than half of the genes have a particular amino acid at that position, otherwise, it is lowercase. Gaps are marked with dashes. The calculated sequence conservation is presented as a bar diagram. (D) Enriched motifs in the B3 domain of REM and other subfamilies. The numbers in brackets manifest the motif sequences shown in Table S4. The size of the numbers shows the frequency of motifs from genes. Colors display the result of the enrichment test for REM and other families (p < 0.0001). (E) The Gene Ontology (GO) term for updated B3 genes. The GO categories are listed below the heatmap