Fig. 1

SSF interacts with SKB1 in nucleus. a SSF interacts with SKB1 in yeast cells. The interaction between T and P53 was used as positive control; pGBKT7 with SSF and SKB1 with pGADT7 were used negative control. b, c BiFC experiments using YFP (b) and split Luciferase (c) show that SSF interacts with SKB1 in Arabidopsis protoplast (b) and in N. benthamiana (c). d Co-localization of SSF and SKB1 in Arabidopsis protoplasts. Scale bar, 10 μm. All these experiments were repeated at least three times and the consistent results were shown in the figure