Fig. 6

Wild-type A. thaliana plant susceptibility to repeated virus stresses. a Experimental design. Batches of 88 three-week-old plants were inoculated with the stimulating viruses TRV, TMV, ROS-tagged TuMV, or mock-inoculated, and 26 - 28 h later, the inoculated leaves were removed to prevent virus spread. Three days after leaf removal, stimulated plants were either challenged with TuMV (n = 78) or mock-inoculated (n = 10). A control batch of plants was also challenged with TuMV after being left without any manipulation (mocking or leaf removal) during the stimulation step. b The number of days it took each plant to develop strong symptoms (all rosette leaves with clear yellowing symptoms) following TuMV inoculation. The stimulation condition included the following treatments performed prior to leave removal: mock inoculation, no manipulation (-), or inoculation with TRV, TMV, or ROS-tagged TuMV. TuMV was inoculated three days after leaf removal in all cases. The results are based on six experimental replicates, each with 88 plants for each condition. Pairwise t-tests: ***P < 0.01; **P < 0.05. c Experimental replicate illustrating a significant visual difference in symptomatology between unstimulated and TRV-, TMV-, or TuMV-stimulated plants 21 days after TuMV challenge