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Table 3 Cell viability test (n = 2) of each strain of Methylobacterium spp. against two concentrations of pure glyphosate (GLY), with and without Tween20 (polysorbate-20). Negative control involved nutrient-rich trypic soy broth (TSB) with no addition of glyphosate or Tween20

From: Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium

Species

Strain

Cell Viability

Neg. Control

2% Tween20

GLY 0.05%

GLY 0.1%

GLY 0.05% + 2% Twen20

GLY 0.1% + 2% Twen20

M. extoquens

JCM 2805

+ + +

+ +

+ + +

+ + +

+ +

+

JCM 2806

+ + +

+ +

+ + +

+ + +

+ +

−

NBRC 103129

+ + +

+ +

+ + +

+ + +

+ +

+

M. gnaphali

NBRC 107716

+ + +

+ +

+ + +

+ + +

+ +

+

M. iners

JCM 16407

+ + +

+ +

+ + +

+ + +

+ +

+

M. jeotgali

LMG 23639

+ + +

+ +

+ + +

+ + +

+ +

−

M. organophilum

NBRC 103119

+ + +

+ +

+ + +

+ + +

+

+

NBRC 103121

+ + +

+ +

+ + +

+ + +

+ +

+

M. oryzae

LMG 23502

+ + +

+ +

+ + +

+ + +

+ +

−

M. oxalidis

NBRC 107715

+ + +

+ +

+ + +

+ + +

+

+

M. phylospharae

LMG 24361

+ + +

+ +

+ + +

+ + +

+ +

−

M. radiotolerans

LMG 6379

+ + +

+ +

+ + +

+ + +

+ +

−

M. rhodinum

LMG 2275

+ + +

+ +

+ + +

+ + +

+ +

+

M. thiocyanatum

NBRC 103128

+ + +

+ +

+ + +

+ + +

+ +

+

JCM 10893

+ + +

+ +

+ + +

+ + +

+ +

+

M. zatmanii

LMG 6087

+ + +

+ +

+ + +

+ + +

+ +

−

Methylobacterium spp.

DSM 23935

+ + +

+ +

+ + +

+ + +

+ +

−

JCM 14674

+ + +

+ +

+ + +

+ + +

+ +

−

E. coli

NM 522

+ + +

+ +

+ + +

+ + +

+ + +

+ + +

  1. (−) no CFU, (+) if < 30 CFU, (+ +) if > 30 CFU or partial lawn, and (+ + +) if complete lawn present and CFU count impossible