Table 1 List of primers used in this study
Gene names | Primer sequences (5′ → 3′) | Application |
|---|---|---|
MaCPR1- Forward | CGGGATCCATGCTGGTCAAGGACGAAG | CDS cloning |
MaCPR1—Reverse | GCGTCGACCCAGACATCTCTCAGATATCGTC | CDS cloning |
MaCPR2- Forward | CCCAAGCTTGGATGAAGGCGGTGGAGCTTCTC | CDS cloning |
MaCPR2—Reverse | GCCCTCGAGCCACACATCACGTAGATACCTGCC | CDS cloning |
MaCPR2- Forward-Trp | ATTTGCGGCCGCATGGACTCCTCGTCGTC | Yeast expression |
MaCPR2—Reverse-Trp | ATTTGCGGCCGCTCACCACACATCACGTAGAT | Yeast expression |
MaC3'H—Forward-Trp | CGGGATCCATGGATCTCCTTCTAATCATTCC | Yeast expression |
MaC3'H—Reverse-Trp | CCCTGAGTTACACATCCACAACCTCGC | Yeast expression |