Fig. 4

Accumulation of an eriodictyol hexoside in A. thaliana 2-ODD mutants carrying the f3h allele. (A) Seedlings stained in situ with diphenylboric acid 2-aminoethyl ester (DPBA) and visualised under UV light. Quercetin derivatives appear orange, kaempferol derivatives green, and blue corresponds to sinapic acid derivatives. (B)In situ DPBA-stained silique of the f3h/fls1/ans mutant visualised under UV light. (C) Accumulation of flavonol- and dihydroflavonol glycosides in siliques analysed by HPTLC and visualised under UV light after treatment with DPBA. The loading to each lane corresponds to methanolic extracts from 3 mg fresh weight of siliques. Kaempferol derivatives are green, quercetin derivatives are orange and sinapic acid derivatives are blue. G, glucose; K, kaempferol; Q, quercetin; R, rhamnose; DHK-D, dihydrokaempferol derivative; DHQ-D, dihydroquercetin derivative; SM, sinapoylmalate; SG, sinapoylglucose; SC, sinapoylcholin. Variations in SG and SC levels are not due to the different genotypes, but to small variations in the developmental stage of the siliques analysed[56]. (D) Eriodictyol standards analysed by HPTLC and visualised under UV light after treatment with DPBA. E, eriodictyol. (E) Eriodictyol and (F) quercetin-3-O-rhamnoside contents in siliques analysed by UPLC-MS/MS MRM. Error bars indicate the standard deviation of four independent measurements. The original, unprocessed versions of Fig. 4C and D can be found in Supplementary Figure S13