Fig. 4

FISH mapping of VvSat67 and VvSat214 repeats on the meiotic pachytene chromosome of Falanghina (FAL), Aglianico (AGL) and Greco Bianco (GRC) grapes. Black and white images of DAPI stained pachytene chromosomes of (A1) FAL; (B1) AGL; and (B1) GRC. Middle column: Signals derived from VvSat67 (green) and VvSat214 (red). Third column: Merged images. Arrowheads point to VvSat214 signals (in red) that are located on a different chromosome from VvSat67 signals (green). Arrows (in A1–3 and B1–3) point to VvSat67 and VvSat214 signals that co-localize on the same chromosome. Scale bars = 5 μm