Fig. 4

Identification and characterization of the cm784 mutant. A Morphological comparison of Baxijiao and cm784 seedlings. Scale bar = 15 cm. B Comparison of Pseudo-stem height, diameter, and Leaf ratio (ratio of the length of the longest leaf to the length of the stem) in seedlings after 45 days of growth in pots. Statistical significance assessed by ANOVA with Turkey’s post hoc test. C Conductivity of leaf at various temperatures between Baxijiao and cm784. The same letter indicates no significant difference at p < 0.05(Turkey’ s test). D Comparison effects of EMS/L-Hyp treatment on the changes of stomatal opening rate between wild type (Baxijiao) and mutation type (cm784). Leaf samples from both the wild-type (WT) and mutant (MT) plants were observed under a microscope, with each visual field equivalent to 10 μm (Bar scales = 10 μm). Insets showing high-magnification views of the boxed areas are shown on the right (Bar scale = 100 μm). E Comparisons of stomatal length; stomatal width; stomatal density and Spongy tissue length between WT and MT. Asterisks indicate significance in paired Tukey test, *P < 0.05, **P < 0.01, ***P < 0.001