Table 1 Bursaphelenchus xylopilus proteomics scientific publications

[99]

To identify differentially expressed surface coat proteins of B. xylophilus during host pine infection and culture in vitro

Surface coat proteins

Reversed-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) followed by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis (MALDI-TOF/MS)

–––––

[100]

To identify the target protein of a specific monoclonal antibody; diagnosis biomarker

Target protein for a specific monoclonal antibody

2-D nano liquid chromatography electrospray ionization quadrupole ion trap tandem mass spectrometry (nano-LC-ESI-Q-IT-MS/MS); data collected in information-dependent acquisition (IDA)

–––––

[101]

To obtain B. xylophilus secretome profile

Secretome

Nano LC–MS/MS; data collected in IDA

–––––-

[102]

To identify population biomarkers

Proteome

Labelled iTRAQ; 2D-nano LC–ESI–MS/MS analysis; absolute quantification

PXD003129

[103]

To compare B. xylophilus and B. mucronatus secretomes; identify pathogenicity biomarkers

Secretome

Non-label short-GeLC, data collected in IDA and comparative quantitative analysis by sequential window acquisition of all theoretical mass spectra (SWATH-MS)

––––––

[104]

To compare B. xylophilus secretomes under pine tree stimuli with different susceptibility to PWD; identify pathogenicity biomarkers

Secretome

Non-label short-GeLC, data collected in IDA and comparative quantitative analysis by SWATH-MS

PXD024011

[105]

To compare secretomes of B. xylophilus isolates with different virulence; identify virulence determinants

Secretome

2D-HPLC and SDS-PAGE; comparative quantitative analysis by gel bands intensities; followed by nano LC–MS/MS of selected bands; data collected in IDA

–––––

[51]

To compare secretomes and proteomes of B. xylophilus isolates with different virulence; identify pathogenicity biomarkers

Secretome and proteome

Non-label short-GeLC, data collected in IDA and comparative quantitative analysis by SWATH-MS

PXD029377