Fig. 5

Gene expression and pathway enrichment in transgenic plants and WT plants. (a) Venn diagram of DEGs of the comparison between transgenic plants and WT plants. (b) Expression profiles derived from RNA-seq are depicted in the cluster; showcasing expression patterns for a subset of genes characterized by high log2 (fold change, FC) values in comparisons between transgenic plants and WT plants, each sampled in triplicate. (c) Distribution and expression levels of differentially expressed genes (DEGs) of overexpression lines and WT plants, the log2 (FC) based on the difference between groups was taken as the abscissa, the negative value of the logarithm based on the difference significance test Q-value value of 10-log10 (q) is the ordinate. (d) Distribution and expression levels of DEGs of SRDX lines and WT plants. (e) Most enriched Gene Ontology (GO) terms of DEGs between CmHRE2-like overexpression lines SRDX lines. The length of the X-axis column corresponds to the size of the Q-value value, while the position on the line above the X-axis represents the count of DEGs annotated to the GO term. (f) KEGG pathway analysis of DEGs between CmHRE2-like overexpression lines and SRDX lines. The graph features the enrichment ratio on the X-axis and the KEGG Pathway on the Y-axis. Bubbles’ sizes convey the number of genes annotated to a KEGG Pathway, while color intensity reflects the enrichment Q-value, with deeper colors denoting lower Q-value value. The copyright for KEGG images has been obtained through written permission from Kanehisa Laboratories