Fig. 6

Metabolomic analysis of ripe L. barbarum fruits harvested at four different periods. A and B PCA score plots of metabolite profiles of L. barbarum fruits harvested four different periods. t[1] represents the principal component 1, t[2] represents the principal component 2, the ellipse represents 95% confidence interval. Points of the same color indicate each biological repeat within the group, the distribution of points reflects the degree of difference between and within groups. The aggregation degree of QC samples reflects whether repeatability of the experiment is good or bad. C and D Venn diagram showing numbers of DAMs in three comparison groups. E The proportion of metabolites identified in L. barbarum fruit in each chemical classification. Different color blocks express different chemical classification items, the percentage represents the number of metabolites in the chemical classification items as a percentage of the total number of identified metabolites. Metabolites without chemical classification are defined as undefined. F Cluster heat map analysis of 8 identical DAMs from three comparison groups. A, C is positive ion mode and B, D is negative ion mode, QC represents the quality control