Variation of antibacterial and antioxidant secondary metabolites and volatiles in leaf and callus extracts of Phulai (Acacia Modesta Wall.)

Table 3 In vitro antibacterial activity and minimum inhibitory concentration (MIC) values of A. modesta leaf and callus extracts

Pathogenic bacteria	Leaf extract		Callus extract		DMSO (negative control)	Ampicillin (positive control)
Pathogenic bacteria	Inhibition zone (mm)	MIC (mg/mL)	Inhibition zone (mm)	MIC (mg/mL)	Inhibition zone (mm)
Escherichia coli	20 ± 0.0^b	1 ± 0.0^a	25 ± 0.7^a	0.5 ± 0.28^b	0.0 ± 0^d	14 ± 0.1^c
Enterobacter cloacae	18 ± 1.4^b	1 ± 0.002^a	26 ± 0.7^a	0.5 ± 0.0^b	0.0 ± 0^d	15 ± 0.0^c
Klebsiella pneumoniae	24 ± 1.4^b	1 ± 0.01^a	35 ± 0.7^a	0.25 ± 0.14^b	0.0 ± 0^d	15 ± 0.0^c
Staphylococcus aureus	20 ± 0.0^b	0.5 ± 0.0^a	37 ± 0.7^a	0.25 ± 0.14^b	0.0 ± 0^c	19 ± 1.4^b
Micrococcus luteus	15 ± 1.4^b	1 ± 0.14^a	25 ± 0.0^a	0.5 ± 0.0^b	0.0 ± 0^c	23 ± 0.7^a

The values are the means of three replicates ± standard errors
The superscript small letters indicate a significant difference at P ≤ 0.05
^a,bIndicate the presence of significant differences between leaf and callus extracts

ISSN: 1471-2229