Skip to main content
Fig. 5 | BMC Plant Biology

Fig. 5

From: Fine mapping and identification of two NtTOM2A homeologs responsible for tobacco mosaic virus replication in tobacco (Nicotiana tabacum L.)

Fig. 5

Functional identification of NtTOM2A under TMV infection. a The sgRNA sequence is highlighted in red and the protospacer-adjacent motif (PAM) is highlighted in green. b, c The NtTOM2A_T and NtTOM2A_S mutation sites of three different mutants and their corresponding amino acid sequences. d Phenotypes of three different mutants 14 days after infection with TMV. e The disease grade of wild type and three different mutants. f Virus accumulation in the apical non-inoculated leaves of the three mutants and wild type. Virus contents were detected by Western blot using an anti-TMV coat protein antibody. The target blot was cropped from the full-length original blot. β-ACTIN: anti-plant actin mouse monoclonal antibody

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us