Fig. 1

Phenotypic characterization of two different cultivars that responded to tobacco mosaic virus (TMV) infection. a Plant phenotype of the susceptible cultivar K326 and resistant cultivar JT88 after infection with TMV-U1 and phosphate buffer saline (MOCK), respectively. Scale bar = 2 cm. b Disease index (DI) of the susceptible cultivar K326 and resistant cultivar JT88 investigated at 14 dpi. c TMV CP relative expression levels in apical non-inoculated leaves (AL) of two different cultivars detected by qRT-PCR. The tobacco actin gene used as an internal reference gene. d TMV accumulation levels in the inoculated leaves (IL) and apical non-inoculated leaves (AL). Virus contents detected by Western blot with an anti-TMV coat protein antibody. The target blot was cropped from the full-length original blot. dpi: days post inoculation, MOCK: mock-inoculated with phosphate buffer saline, TMV CP: TMV coat protein, β-ACTIN: anti-plant actin mouse monoclonal antibody. The data (Fig. 1b and 1c) are presented as means ± SDs of three biological replicates. Student t-tests were used to determine the significance of differences between K326 and JT88 (****P < 0.0001)