Fig. 5

FISH localization of five centromeric satellite repeats on mitotic metaphase chromosomes of RO1 accession. (a) Dual-color FISH of AeSat-B (red) and AeSat-E (green), showing colocalization of these two satellite repeats on the chromosomes of RO1; (b) Dual-color FISH of AeSat-B (red) and AeSat-A (green), showing non-colocalization of these two satellite repeats on the chromosomes of RO1; (c) Dual-color FISH of AeSat-B (red) and AeSat-C (green), showing non-colocalization of these two satellite repeats except on one pair of chromosomes of RO1; (d) Dual-color FISH of AeSat-B (red) and AeSat-D (green), showing colocalization of these two satellite repeats on a few chromosomes of RO1. Thus, five centromeric satellite repeats exhibit distribution bias within the two subgenomes in RO1 accession. Scale bars: 1 μm