Fig. 5

The impact of salt and osmotic stress on the growth of wild type, at5g58150, and 35S::AT5G58150-GFP seedlings. A, B Germination of seeds sown on media with either 100 mM NaCl or 200 mM NaCl or with no additional solute. The experiment was repeated 5 times, with 52 seeds per group. C, D Germination of seeds sown on media with either 200 mM mannitol or 400 mM mannitol or without any component added. The experiment was repeated seven times, with 52 seeds per group. In A–D, seeds were classified into three groups: (i) non-germinated, (ii) with visible radicle and cotyledons, if visible, unopened, (iii) with radicle visible and opened cotyledons, on the second (A, B) and third (C, D) day of growth. Error bars represent SE. Asterisks mark groups that differ in log-odds ratios from wild-type control grown on the same medium (likelihood ratio χ² test, *p = 0.01–0.05; **p = 0.001–0.01, ***p < 0.001, adjusted with Hommel’s method). E-G Primary root length (E), lateral root number (F) and lateral root density (G) of 9-day-old seedlings growing on the media with 100 mM NaCl or 200 mM mannitol or no solute added. Lateral root density was calculated as the number of lateral roots per cm of the primary root. The experiment was repeated 3 times, with 48 seeds per group. Asterisks mark groups that differ in mean from wild-type control grown on the same medium (*p = 0.01–0.05; **p = 0.001–0.01, ***p < 0.001, adjusted with Hommel’s method). H The impact of salt and osmotic stresses on the promoter activity of 35S::GUS, and AT5G58150::GUS seedlings. Seeds were sown on media either with 100 mM NaCl or 200 mM mannitol or without added components. After 7 days, the promoter activity was visualised with GUS staining. Each experiment was repeated five times, with 52 seeds sown per group. I AT5G58150 expression pattern in seedlings grown in control conditions (left picture) and the presence of 200 mM mannitol (middle picture) or 100 mM NaCl (left) added to the growth media after 8 days of germination. The experiment was repeated three times