Fig. 4

Interactions between AT5G58150 and PHOT2 examined with the Bimolecular Fluorescence Complementation Method. A Confocal images of N. benthamiana epidermal cells transiently co-expressing PHOT2 and AT5G58150 fused with C(N)-terminal YFP fragments in the following configurations: AT5G58150__NtermYFP and AT5G58150__CtermYFP, NtermYFP_PHOT2 and PHOT2__CtermYFP, _NtermYFP_PHOT2 and AT5G58150__CtermYFP, _CtermYFP_PHOT2 and PHOT2__NtermYFP, PHOT2__CtermYFP and AT5G58150__NtermYFP. Chlorophyll autofluorescence is in magenta and reconstituted YFP fluorescence is in green. The results represent one of four independent biological replicates. B Western Blot analysis of N. benthamiana epidermal cells transiently co-expressing PHOT2 and AT5G58150 fused with C(N)-terminal YFP fragments in the following configurations: AT5G58150__NtermYFP and AT5G58150__CtermYFP, _NtermYFP_PHOT2 and PHOT2__CtermYFP, _NtermYFP_PHOT2 and AT5G58150__CtermYFP, _CtermYFP_PHOT2 and PHOT2__NtermYFP, PHOT2__CtermYFP and AT5G58150__NtermYFP, AT5G58150__NtermYFP, AT5G58150__CtermYFP, _NtermYFP_PHOT2, _CtermYFP_PHOT2, PHOT2__NtermYFP, PHOT2__CtermYFP probed with anti-cYFP and anti-nYFP. The lower image shows the same blot after stripping and probing with anti-actin antibodies. Uncropped image in Fig. S5. The results represent one of three independent biological replicates