Fig. 2
From: Phototropin2 3’UTR overlaps with the AT5G58150 gene encoding an inactive RLK kinase
PHOT2 transcript (A, B, C) and protein (D, E, F) relative expression levels in wild type, at5g58150, 35S::AT5G58150-GFP, and phot2 plants. A, D Dark-adapted overnight, 5-week-old mature Arabidopsis leaves were irradiated with blue or red light of 120 µmol·m−2·s−1 for 3 h or kept in darkness. B, C, E, F Seedlings were grown in vitro in the presence of 100 mM NaCl, 200 mM, and 400 mM mannitol or without any supplementation on the B5 medium for 1 week. In A–F each bar corresponds to an average of measurements from four biological replicates. Error bars indicate the standard error. For any pair of bars, their means differ at the significance level of 0.05 if and only if they do not share a letter (tested with the Tukey method). G 3'UTR sequences of PHOT2 and AT5G58150. Blue capital letters: coding sequences (CDS) of PHOT2 (top) and AT5G58150 (bottom), black: 3' UTRs of PHOT2/AT5G58140 (top) and AT5G58150 (bottom), bold: a 66 bp long overlap of PHOT2 and AT5G58150 3'UTRs, in red: stop codons. Arrows mark the 5' to 3' direction