Fig. 8

(A) The determination of the content of the four compounds (loganic acid, sweroside, gentiopicroside and swertiamarin) in the seedlings of G. macrophylla treated with MeJA. The red columns represent the content determination results of G. macrophylla seedlings treated with MeJA. The blue columns represent the content determination results of G. macrophylla seedlings without MeJA treatment (Control). The asterisks (*) represent significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001; and ****, P < 0.0001). (B) Chromatograms for the determination of the content of four compounds in G. macrophylla seedlings for 3 days. The red chromatogram shows the content determination chromatogram of G. macrophylla seedlings treated with MeJA. The blue chromatogram shows the content determination of G. macrophylla seedlings without MeJA treatment (Control). 1: Loganic acid, 2: Swertiamarin, 3: Gentiopicroside, 4: Sweroside