Fig. 5

Analysis of gene expression involved in the secoiridoid biosynthesis of G. macrophylla. Different color blocks represent the normalized gene expression levels (log2 (FPKM)) of all genes in different tissues. The yellow ellipse represents the MVA pathway, the light blue ellipse represents the MEP pathway, and the green ellipse represents the characteristic secoiridoid pathway. GmAACT, acetoacetyl-CoA thiolase; GmHMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; GmHMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; GmMK, mevalonate kinase; GmPMK, phosphomevalonate kinase; GmMDC, mevalonate-5-pyrophosphate decarboxylase; GmDXS, deoxyd-xylulose-5-phosphate synthase; GmDXR, deoxy-D-xylulose-5-phosphate reductoisomerase; GmCMS, 4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol synthase; GmCMK, 4-(cytidine 5’-diphospho)-2-C-methyl-D-erythritol kinase; GmMCS, 2-C-methyld-erythritol2,4-cyclodiphosphate synthase; GmHDS, 4-hydroxy-3-methylbut-2-enyl diphosphate synthase; GmHDR, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase; GmIDI, isopentenyl diphosphate isomerase; GmGPPS, geranyl diphosphate synthase; GmFPPS, farnesyl diphosphate synthase; GmGGPPS, geranylgeranyl diphosphate synthases; GmGES, geraniol synthase; GmG10H geraniol 10-hydroxylase; Gm8-HGO, 8-hydroxy-geraniol oxidoreductase; GmIS, iridoid synthase; GmNS, nepetalactol synthase; GmIO, iridoid oxidase; Gm7-DLGT, 7-deoxyloganetic acid glucosyltransferase; Gm7-DLNGT, 7-deoxyloganetic glucosyltransferase; Gm7-DLH, 7-deoxyloganate 7-hydroxylase; and GmSLS, secologanin synthase; were recorded as the key enzymes in the pathway