Fig. 5

Contents of terpenoids in hairy roots of McFPS detected using GC–MS A Transgenic hairy roots of German chamomile. B Electrophoresis of rolB and rolC from transgenic hairy roots. C qPCR analysis of McFPS transgenic hairy roots. D–H Analysis of volatile components in McFPS transgenic hairy roots by using GC–MS (D, δ-selinene and β-neoclovene; E, α-guaiene; F, γ-muurolene; G, β-santalol; H, cis-β-farnesene) Note: M: DNA marker; 1–5: rolB electrophoresis of transgenic hairy roots; 6–10: rolC electrophoresis of transgenic hairy roots. The 18S gene was used as the reference gene. The 2^−ΔCt method was used to calculate relative expression levels