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Fig. 6 | BMC Plant Biology

Fig. 6

From: Genome-wide analysis of UDP-glycosyltransferase gene family and identification of a flavonoid 7-O-UGT (AhUGT75A) enhancing abiotic stress in peanut (Arachis hypogaea L.)

Fig. 6

Enzymatic assay and subcellular localization of AhUGT75A. A SDS-PAGE analysis of purified recombinant AhUGT75A. The original uncropped image of protein gel electrophoresis was shown in Fig. S8. B In vitro enzyme activities of recombinant AhUGT75A toward various flavonoids with the presence of UDP-glucose. C HPLC analysis of the products from the reaction catalyzed by recombinant AhUGT75A using naringenin, liquiritigenin or hesperetin as substrate. CK was negative control reaction without protein. D Mass spectrum identification of glucosylation products of enzyme reactions. E Subcellular localization analysis of AhUGT75A. PC1300-35S::AhUGT75A-GFP or PC1300-35S::GFP empty vector was transformed into Arabidopsis protoplasts expressing a red fluorescent mitochondrion marker (mitochondrion-RFP) using PEG-mediated method, respectively

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BMC Plant Biology

ISSN: 1471-2229

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