Systematic analysis of Heat Shock Protein 70 (HSP70) gene family in radish and potential roles in stress tolerance

Pan, Xiaoxue; Zheng, Yang; Lei, Kairong; Tao, Weilin; Zhou, Na

doi:10.1186/s12870-023-04653-6

Table 1 The general information and sequence characterization of 34 RsHSP70 genes

From: Systematic analysis of Heat Shock Protein 70 (HSP70) gene family in radish and potential roles in stress tolerance

S.N	Gene^a	Locus^b	Location^c	ORF(bp)^d	Exon^e	Protein^f			Subcellular location^g
S.N	Gene^a	Locus^b	Location^c	ORF(bp)^d	Exon^e	Size (aa)	MW(d)	pI	Subcellular location^g
1	RsHSP70-1	Rsa10038515	R01:6,368,319–6,370,518	1692	1	563	60,475.48	5.27	cyto: 8, chlo: 5
2	RsHSP70-2	Rsa10012256	R01:32,783,050–32786226	1908	4	635	68,894.74	4.73	cyto: 9, cysk: 3, chlo: 1
3	RsHSP70-3	Rsa10034690	R01:48,749,418–48,750,437	1023	1	340	36,918.8	4.49	cyto: 8, nucl: 2.5, cysk_nucl: 2, chlo: 1, mito: 1
4	RsHSP70-4	Rsa10021747	R02:6,150,715–6157881	4008	11	1335	144,259.7	8.32	chlo: 8, mito: 2, vacu: 2, plas: 1
5	RsHSP70-5	Rsa10021746	R02:6,158,811–6,162,178	1695	8	564	60,435.85	4.4	cyto: 10, mito: 2, pero: 2
6	RsHSP70-6	Rsa10018910	R02:9,695,473–9,699,355	2700	14	899	99,811.07	5.47	chlo: 4, nucl: 3, plas: 3, cyto: 1, vacu: 1, E.R.: 1
7	RsHSP70-7	Rsa10024964	R02:12,928,642–12,933,512	2052	6	683	73,014.51	5.58	mito: 10, chlo: 4
8	RsHSP70-8	Rsa10030573	R02:37,598,054–37599295	1245	1	414	47,290.77	5.16	cyto: 5, nucl: 5, plas: 1, cysk: 1, golg: 1
9	RsHSP70-9	Rsa10036337	R03:1,667,438–1,673,472	2052	5	683	72,750.21	5.34	mito: 12, chlo: 2
10	RsHSP70-10	Rsa10032304	R03:8,248,787–8,251,621	2046	6	681	72,578.1	4.83	E.R.: 7, chlo: 3, cyto: 2, extr: 1
11	RsHSP70-11	Rsa10036631	R04:4,165,044–4167982	2139	8	712	76,140.85	5.13	chlo: 14
12	RsHSP70-12	Rsa10025332	R04:6,821,393–6,825,331	2691	14	896	99,731.31	6.07	E.R.: 6, chlo: 4, vacu: 2, cyto: 1
13	RsHSP70-13	Rsa10005413	R04:49,330,589–49334805	2445	9	814	90,108.18	4.89	cyto: 9, nucl: 3, chlo: 2
14	RsHSP70-14	Rsa10042765	R05:2,174,643–2,176,040	717	1	238	25,867.96	4.46	cyto: 6, mito: 5, chlo: 1, nucl: 1
15	RsHSP70-15	Rsa10041785	R05:7,596,122–7,598,778	1956	2	651	71,190.63	4.78	cyto: 8, cysk: 4, chlo: 1
16	RsHSP70-16	Rsa10019803	R05:16,754,020–16756483	1896	5	631	69,998.34	4.68	cyto: 13
17	RsHSP70-17	Rsa10010269	R05:27,677,825–27,680,504	2067	6	688	72,115.42	4.72	E.R.: 7, chlo: 3, cyto: 2, extr: 1
18	RsHSP70-18	Rsa10009146	R06:1,006,847–1010066	2454	9	817	90,061.33	4.92	cyto: 9, chlo: 3, nucl: 2
19	RsHSP70-19	Rsa10010766	R06:4,867,783–4,868,904	1125	1	374	42,038.14	5.4	nucl: 6, cyto: 6, mito: 1
20	RsHSP70-20	Rsa10039994	R06:16,654,115–16,655,488	1377	1	458	50,374.95	4.72	cyto: 8, chlo:6
21	RsHSP70-21	Rsa10038047	R06:20,575,035–20578239	1947	1	648	71,048.53	5.33	cyto: 13
22	RsHSP70-22	Rsa10037733	R06:25,576,889–25,580,215	2271	9	756	83,993.89	5.92	nucl: 10, chlo: 1, cyto: 1, vacu: 1
23	RsHSP70-23	Rsa10031033	R07:2,236,711–2,239,183	1959	5	652	70,528.96	4.79	E.R.:5, chlo: 4, cyto.: 2, extr: 2
24	RsHSP70-24	Rsa10023626	R07:12,631,430–12632851	1425	1	474	52,005.73	4.7	cyto: 7, chlo: 6
25	RsHSP70-25	Rsa10007904	R07:25,365,226–25,367,405	1944	2	647	70,846.34	4.81	cyto: 9, cysk: 3, chlo: 1
26	RsHSP70-26	Rsa10010304	R08:17,739,581–17,741,005	1428	1	475	52,154.89	4.67	cyto: 7, chlo: 6
27	RsHSP70-27	Rsa10026855	R08:18,902,782–18903463	549	2	182	20,604.26	6.81	mito: 7, cyto: 6.5, nucl: 4
28	RsHSP70-28	Rsa10026857	R08:18,911,557–18,914,418	2043	8	680	72,260.14	4.85	chlo: 14
29	RsHSP70-29	Rsa10026985	R08:19,773,591–19,775,985	1986	6	661	65,334.75	8.85	mito: 12, chlo: 2
30	RsHSP70-30	Rsa10026987	R08:19,779,354–19,784,550	3510	11	1169	125,986.3	7.44	chlo: 8, mito: 5
31	RsHSP70-31	Rsa10040958	R08:24,052,015–24054747	1968	1	655	71,607.85	5.05	cyto: 11, chlo: 2
32	RsHSP70-32	Rsa10023846	R09:27,216,803–27217385	339	3	112	12,208.96	8.21	chlo: 11, cyto: 1, plas: 1
33	RsHSP70-33	Rsa10015012	R09:33,292,486–33,293,068	414	3	137	15,093.08	5.69	chlo: 7, mito: 3, nucl: 2, cyto: 1
34	RsHSP70-34	Rsa10002306	Scaffold326:1636–3069	1437	1	478	52,452.2	4.67	cyto: 7, chlo: 5, E.R.: 1

^a Systematic designation given to radish HSP70s in this study
^b Locus identity number of RsHSP70s assigned by BRAD database (http://brassicadb.org/brad/)
^c Chromosomal localization of radish HSP70 genes
^d Length of the open reading frame
^e Number of extrons obtained from GSDS by comparing sequences between transcript and genome (Gene Structure Display Server; http://gsds.cbi.pku.edu.cn/)
^f Protein characterization of RsHSP70s obtained from EXPASY server (http://web.expasy.org/protparam/)
^g Subcellular localization of each HSP70 protein obtained from WoLF PSORT (https://wolfpsort.hgc.jp/). chlo, chloroplast; cyto, cytosol; nucl, nucleus; E.R., endoplasmic reticulum; mito, mitocondria; plas, plasma membrane; extr, extracelular; cysk, cytoskeleton; pero, peroxisome; vacu, vacuolar membrane; golg, Golgi S.N., serial number; ORF, open reading frame; bp, base pair; aa, amino acids; MW, molecular weight; pI, isoelectric point

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