Fig. 3

Karyotyping for different ploidy potatoes. Molecular karyotypes of At (A), and EA49 (B) with 1× sequence depth. The scatter represents Ratio * expected ploidy (4 for At; 6 for EA49), and the black line shows the copy number of chromosomes. (C) Genotyping of the EA49 with 10× sequence depth. Using S. etubersoum (EE) as the reference parent to calculate the index, and the scatter represents the index of each position. The black line, obtained by sliding window analysis, represents the proportion (2/3, black dotted line) of AC142 (AA) genotypes. The red line is “1 - black line”, representing the ratio (1/3, red dotted line) of EE genotypes. (D) FISH mapping of At chromosomes using two oligo-FISH probes (lanes A and C). The same cell was reprobed with subtelomeric repeated sequences CL34 (green), CL14 (red), and 45 S rDNA (white) probes (lanes B and D). At is an autotetraploid with 48 chromosomes. (E) GISH mapping of EA49 chromosomes using two genomic probes (AA, green; EE, red). EA49 is a hexaploid with 72 chromosomes consisting of two sets of A genomes and one set of E genome. Scale bar, 10 μm