Fig. 2
From: Karyotyping of aneuploid and polyploid plants from low coverage whole-genome resequencing
Karyotyping for different ploidy rapeseeds. (A) For molecular karyotypes of ESS1_17 with 1× sequence depth, the scatter represents Ratio * 2 (expected ploidy). (B) For molecular karyotypes of 21A020 with 1× sequence depth, the scatter represents Ratio * expected ploidy (2 for subgenome A; 1 for subgenome C). The black line shows the copy number of chromosomes. (C-D) For cytogenetic karyotype analysis, the A chromosomes are shown in lanes A and B, and the C chromosomes are shown in lanes C and D. The first round of FISH included 45 S rDNA (white), 5 S rDNA (yellow), BAC clone KBrB072L17 (green), and KBrH092N24 (red) probes, and the hybridization results are shown in lanes A and C. The second round of FISH included CentBr1 (white), CentBr2 (green), and BAC BNIH 123L05 (red) probes containing C genome-specific repeated sequences, and the hybridization results are shown in lanes B and D. (C) A MAAL ESS1_17 with two sets of A genomes plus one more C3 chromosome. (D) An allotriploid 21A020 is composed of two sets of A genomes and one set of C genome