Fig. 1
From: Karyotyping of aneuploid and polyploid plants from low coverage whole-genome resequencing
Karyotyping of the resynthesized B. napus QIS4_8. (A) Molecular karyotype of the QIS4_8 with 1× sequence depth. The scatter represents Ratio * 2 (expected ploidy), and the black line shows the copy number of chromosomes. (B, C, and D) Genotyping of the QIS4_8 with different sequence depths (10, 5, and 1×). The scatter represents the index of each position. The black line was obtained by sliding window analysis. The two red dotted lines divide the average index (black line) into three regions as previously described [30]. Thus, 0-0.2 (variation type from Quinta) and 0.8-1 (IMB218 alleles) are homozygous regions, and 0.2–0.8 is the heterozygous region. (E) For cytogenetic karyotype analysis, the A chromosomes are shown in lanes A and B, and the C chromosomes are shown in lanes C and D. The first round of FISH included 45 S rDNA (white), 5 S rDNA (yellow), BAC clone KBrB072L17 (green), and KBrH092N24 (red) probes, and the hybridization results are shown in lanes A and C. The second round of FISH included CentBr1 (white), CentBr2 (green), and BAC BNIH 123L05 (red) probes containing C genome-specific repeated sequences, and the hybridization results are shown in lanes B and D. One near-tetraploid QIS4_8 with 38 chromosomes derived from the AnArCn allotriploid