Fig. 3

Syntenic relationship of Avena strigosa (AST), A. longiglumis (ALO), and A. eriantha (AER) genomes

A Syntenic analysis of AST (top), ALO (middle), and AER (bottom). Subterminal regions are frequently involved in inter- and intra-specific evolutionary translocations. B Dotplot of AAT-ALO. C–E Fluorescent in situ hybridization (FISH) showing evolutionarily recent inter-genomic translocations between Avena genomes in the polyploid A. sativa. These are similar in extent to those identified by synteny analysis between Avena species. C FISH karyotype of A. sativa. Probes are AF226603_45bp (TET, pseudo-blue) for C genome, pAs120a (biotin, pseudo-red) for A genome, and Ab-T148 (digoxigenin, pseudo-green) for A/D genome. Probes were amplified from A. longiglumis. D Translocation shown by orange dotted square in Fig. 3C. D and E Translocation shown by green dotted square in Fig. 3C